| Literature DB >> 19245789 |
Zhi-Xin Shan1, Qiu-Xiong Lin, Yong-Heng Fu, Chun-Yu Deng, Zhi-Ling Zhou, Jie-Ning Zhu, Xiao-Ying Liu, You-Yi Zhang, Yangxin Li, Shu-Guang Lin, Xi-Yong Yu.
Abstract
MicroRNAs (miRNAs) have been increasingly reported to have important roles in diverse biological and pathological processes. We investigated miR-1 and miR-206 expression and their potential roles in a rat model of myocardial infarction (MI). miR-1 and miR-206 expression were significantly increased, and insulin-like growth factor 1 (IGF-1) protein was markedly reduced without obvious change of its mRNA level after MI induction. Position 175-196 of rat IGF-1 3'-untranslated region was identified to be required for efficient downregulation by miR-1/miR-206. IGF-1 level was reduced without changing its transcript level in rat H9C2 myoblast cells modified with miR-1 (H9C2-miR-1). In the serum withdrawal and hypoxic condition, caspase-3 activity and mitochondrial potential were significantly increased in H9C2-miR-1 cells compared with the control group, respectively (p<0.05, p<0.01). Together, our results indicate that miR-1 and miR-206 are involved in apoptotic cell death in MI by post-transcriptional repression of IGF-1.Entities:
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Year: 2009 PMID： 19245789 DOI： 10.1016/j.bbrc.2009.02.097
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575