Isolation and identification of human hair ceramides.

Synopsis The analysis of the lipids extracted from human hair by silica-gel-column liquid chromatography permitted an isolation of the fraction enriched in free ceramides. These were identified by GC/MS and are related to ceramide classes 2 and 5 in which the long-chain base was predominantly sphinganine. This differs from that observed in the free ceramides found within the stratum corenum.