PURPOSE: To use magnetic resonance (MR) imaging and positron emission tomography (PET) dual detection of cardiac-grafted embryonic stem cells (ESCs) to examine (a) survival and proliferation of ESCs in normal and infarcted myocardium, (b) host macrophage versus grafted ESC contribution to serial MR imaging signal over time, and (c) cardiac function associated with the formation of grafts and whether improvement in cardiac function is related to cardiac differentiation of ESCs. MATERIALS AND METHODS: All animal procedures were approved by the institutional animal care and use committee. Murine ESCs were stably transfected with a mutant version of herpes simplex virus type 1 thymidine kinase, HSV1-sr39tk, and also were labeled with superparamagnetic iron oxide (SPIO) particles. Cells were injected directly in the border zone of the infarcted heart or in corresponding regions of normal hearts in athymic rats. PET and MR imaging were performed longitudinally for 4 weeks in the same animals. RESULTS: ESCs survived and underwent proliferation in the infarcted and normal hearts, as demonstrated by serial increases in 9-(4-[(18)F]fluoro-3-hydroxymethylbutyl) guanine PET signals. In parallel, the hypointense areas on MR images at the injection sites decreased over time. Double staining for host macrophages and SPIO particles revealed that the majority of SPIO-containing cells were macrophages at week 4 after injection. Left ventricular ejection fraction increased in the ESC-treated rats but decreased in culture media-treated rats, and border-zone function was preserved in ESC-treated animals; however, cardiac differentiation of ESCs was less than 0.5%. CONCLUSION: Dual-modality imaging permits complementary information in regard to cell survival and proliferation, graft formation, and effects on cardiac function. SUPPLEMENTAL MATERIAL: http://radiology.rsnajnls.org/cgi/content/full/250/3/821/DC1. RSNA, 2009
PURPOSE: To use magnetic resonance (MR) imaging and positron emission tomography (PET) dual detection of cardiac-grafted embryonic stem cells (ESCs) to examine (a) survival and proliferation of ESCs in normal and infarcted myocardium, (b) host macrophage versus grafted ESC contribution to serial MR imaging signal over time, and (c) cardiac function associated with the formation of grafts and whether improvement in cardiac function is related to cardiac differentiation of ESCs. MATERIALS AND METHODS: All animal procedures were approved by the institutional animal care and use committee. Murine ESCs were stably transfected with a mutant version of herpes simplex virus type 1 thymidine kinase, HSV1-sr39tk, and also were labeled with superparamagnetic iron oxide (SPIO) particles. Cells were injected directly in the border zone of the infarcted heart or in corresponding regions of normal hearts in athymic rats. PET and MR imaging were performed longitudinally for 4 weeks in the same animals. RESULTS: ESCs survived and underwent proliferation in the infarcted and normal hearts, as demonstrated by serial increases in 9-(4-[(18)F]fluoro-3-hydroxymethylbutyl) guanine PET signals. In parallel, the hypointense areas on MR images at the injection sites decreased over time. Double staining for host macrophages and SPIO particles revealed that the majority of SPIO-containing cells were macrophages at week 4 after injection. Left ventricular ejection fraction increased in the ESC-treated rats but decreased in culture media-treated rats, and border-zone function was preserved in ESC-treated animals; however, cardiac differentiation of ESCs was less than 0.5%. CONCLUSION: Dual-modality imaging permits complementary information in regard to cell survival and proliferation, graft formation, and effects on cardiac function. SUPPLEMENTAL MATERIAL: http://radiology.rsnajnls.org/cgi/content/full/250/3/821/DC1. RSNA, 2009
Authors: Jonathan M Hill; Alexander J Dick; Venkatesh K Raman; Richard B Thompson; Zu-Xi Yu; K Allison Hinds; Breno S S Pessanha; Michael A Guttman; Timothy R Varney; Bradley J Martin; Cynthia E Dunbar; Elliot R McVeigh; Robert J Lederman Journal: Circulation Date: 2003-08-11 Impact factor: 29.690
Authors: James B Young; Mark E Dunlap; Marc A Pfeffer; Jeffrey L Probstfield; Alain Cohen-Solal; Rainer Dietz; Christopher B Granger; Jaromir Hradec; Jerzy Kuch; Robert S McKelvie; John J V McMurray; Eric L Michelson; Bertil Olofsson; Jan Ostergren; Peter Held; Scott D Solomon; Salim Yusuf; Karl Swedberg Journal: Circulation Date: 2004-10-18 Impact factor: 29.690
Authors: Rong Zhou; Daniel H Thomas; Hui Qiao; Harshali S Bal; Seok-Rye Choi; Abass Alavi; Victor A Ferrari; Hank F Kung; Paul D Acton Journal: J Nucl Med Date: 2005-05 Impact factor: 10.057
Authors: Joseph C Wu; Ian Y Chen; Gobalakrishnan Sundaresan; Jung-Joon Min; Abhijit De; Jian-Hua Qiao; Michael C Fishbein; Sanjiv S Gambhir Journal: Circulation Date: 2003-09-08 Impact factor: 29.690
Authors: Zequan Yang; Stuart S Berr; Wesley D Gilson; Marie-Claire Toufektsian; Brent A French Journal: Circulation Date: 2004-02-16 Impact factor: 29.690
Authors: Steven N Ebert; David G Taylor; Ha-Long Nguyen; David P Kodack; Ronald J Beyers; Yaqin Xu; Zequan Yang; Brent A French Journal: Stem Cells Date: 2007-08-09 Impact factor: 6.277
Authors: Hualei Zhang; Hui Qiao; Rachel S Frank; Bin Huang; Kathleen J Propert; Susan Margulies; Victor A Ferrari; Jonathan A Epstein; Rong Zhou Journal: Circ Cardiovasc Imaging Date: 2012-02-06 Impact factor: 7.792
Authors: Tommi Kokki; Hannu T Sipilä; Mika Teräs; Tommi Noponen; Nicolas Durand-Schaefer; Riku Klén; Juhani Knuuti Journal: J Nucl Cardiol Date: 2010 Jan-Feb Impact factor: 5.952