Literature DB >> 19243167

Combinatorial synthesis of thrombin-binding aptamers containing iso-guanine.

Subba Rao Nallagatla1, Benjamin Heuberger, Azizul Haque, Christopher Switzer.   

Abstract

A library of all possible substitutions of guanine by iso-guanine (iG) in the thrombin aptamer was prepared by split and mix synthesis. A colorimetric assay was used to screen for functional oligomers in the library. Colorimetrically active oligonucleotides were selected and sequenced by the Maxam-Gilbert method. The sequenced oligonucleotides were individually resynthesized, and their affinities for thrombin were assayed by isothermal titration calorimetry. Three aptamer sequences containing iG were found to have enhanced binding activity to human alpha-thrombin compared to the parent aptamer.

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Year:  2009        PMID： 19243167     DOI： 10.1021/cc800178m

Source DB:  PubMed          Journal:  J Comb Chem        ISSN： 1520-4766


  14 in total

1.  Through-bond effects in the ternary complexes of thrombin sandwiched by two DNA aptamers.

Authors:  Andrea Pica; Irene Russo Krauss; Valeria Parente; Hisae Tateishi-Karimata; Satoru Nagatoishi; Kouhei Tsumoto; Naoki Sugimoto; Filomena Sica
Journal:  Nucleic Acids Res       Date:  2016-11-28       Impact factor: 16.971

Review 2.  In What Ways Do Synthetic Nucleotides and Natural Base Lesions Alter the Structural Stability of G-Quadruplex Nucleic Acids?

Authors:  Janos Sagi
Journal:  J Nucleic Acids       Date:  2017-10-18

3.  Experimental and mathematical evidence that thrombin-binding aptamers form a 1 aptamer:2 protein complex.

Authors:  Kepler S Mears; Daniel L Markus; Oluwadamilare Ogunjimi; Rebecca J Whelan
Journal:  Aptamers (Oxf)       Date:  2018-10-10

4.  Anomeric DNA quadruplexes.

Authors:  Natalia A Kolganova; Anna M Varizhuk; Roman A Novikov; Vladimir L Florentiev; Galina E Pozmogova; Olga F Borisova; Anna K Shchyolkina; Igor P Smirnov; Dmitry N Kaluzhny; Edward N Timofeev
Journal:  Artif DNA PNA XNA       Date:  2014

5.  High-resolution structures of two complexes between thrombin and thrombin-binding aptamer shed light on the role of cations in the aptamer inhibitory activity.

Authors:  Irene Russo Krauss; Antonello Merlino; Antonio Randazzo; Ettore Novellino; Lelio Mazzarella; Filomena Sica
Journal:  Nucleic Acids Res       Date:  2012-06-04       Impact factor: 16.971

6.  Improved thrombin binding aptamer by incorporation of a single unlocked nucleic acid monomer.

Authors:  Anna Pasternak; Frank J Hernandez; Lars M Rasmussen; Birte Vester; Jesper Wengel
Journal:  Nucleic Acids Res       Date:  2010-09-24       Impact factor: 16.971

7.  Direct detection of thrombin binding to 8-bromodeoxyguanosine-modified aptamer: effects of modification on affinity and kinetics.

Authors:  Shou Goji; Jun Matsui
Journal:  J Nucleic Acids       Date:  2011-09-15

8.  Thrombin-aptamer recognition: a revealed ambiguity.

Authors:  Irene Russo Krauss; Antonello Merlino; Concetta Giancola; Antonio Randazzo; Lelio Mazzarella; Filomena Sica
Journal:  Nucleic Acids Res       Date:  2011-06-28       Impact factor: 16.971

Review 9.  Nucleic Acid Aptamers: An Emerging Tool for Biotechnology and Biomedical Sensing.

Authors:  Ti-Hsuan Ku; Tiantian Zhang; Hua Luo; Tony M Yen; Ping-Wei Chen; Yuanyuan Han; Yu-Hwa Lo
Journal:  Sensors (Basel)       Date:  2015-07-06       Impact factor: 3.576

10.  Thermodynamic, Anticoagulant, and Antiproliferative Properties of Thrombin Binding Aptamer Containing Novel UNA Derivative.

Authors:  Weronika Kotkowiak; Jolanta Lisowiec-Wachnicka; Jakub Grynda; Ryszard Kierzek; Jesper Wengel; Anna Pasternak
Journal:  Mol Ther Nucleic Acids       Date:  2017-12-30       Impact factor: 8.886
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