J V Rogers1, W R Richter, M Q Shaw, Y W Choi. 1. Battelle Biomedical Research Center, 505 King Avenue, JM-7, Columbus, OH 43201, USA. rogersjv@battelle.org
Abstract
AIMS: This study evaluated the inactivation of virulent Yersinia pestis dried on polymers, steel, and glass surfaces using vapour-phase hydrogen peroxide. METHODS AND RESULTS: A suspension of Y. pestis CO92 (1.70 x 10(8) CFU) was dried on 10 different types of test surfaces and exposed to vapour-phase hydrogen peroxide fumigation for a contact time of 2 h. A significant reduction in the log10 CFU of Y. pestis on all 10 materials was observed between the controls evaluated after a 1 h drying time and unexposed controls evaluated after the decontamination run. Qualitative growth assessment showed that vapour-phase hydrogen peroxide exposure inactivated Y. pestis on all replicates of the 10 test materials as well as biological indicators up to 7 days postexposure. CONCLUSIONS: Virulent Y. pestis CO92 is inactivated on polymers, steel, and glass surfaces when exposed to vapour-phase hydrogen peroxide without observable physical damage to the test materials. SIGNIFICANCE AND IMPACT OF THE STUDY: This study provides information for using vapour-phase hydrogen peroxide as a practical process for the decontamination of virulent Y. pestis in circumstances where time-dependent attenuation/inactivation orliquid/heat decontamination may not be the most suitable approach.
AIMS: This study evaluated the inactivation of virulent Yersinia pestis dried on polymers, steel, and glass surfaces using vapour-phase hydrogen peroxide. METHODS AND RESULTS: A suspension of Y. pestis CO92 (1.70 x 10(8) CFU) was dried on 10 different types of test surfaces and exposed to vapour-phase hydrogen peroxide fumigation for a contact time of 2 h. A significant reduction in the log10 CFU of Y. pestis on all 10 materials was observed between the controls evaluated after a 1 h drying time and unexposed controls evaluated after the decontamination run. Qualitative growth assessment showed that vapour-phase hydrogen peroxide exposure inactivated Y. pestis on all replicates of the 10 test materials as well as biological indicators up to 7 days postexposure. CONCLUSIONS: Virulent Y. pestis CO92 is inactivated on polymers, steel, and glass surfaces when exposed to vapour-phase hydrogen peroxide without observable physical damage to the test materials. SIGNIFICANCE AND IMPACT OF THE STUDY: This study provides information for using vapour-phase hydrogen peroxide as a practical process for the decontamination of virulent Y. pestis in circumstances where time-dependent attenuation/inactivation orliquid/heat decontamination may not be the most suitable approach.
Authors: Vipin K Rastogi; Shawn P Ryan; Lalena Wallace; Lisa S Smith; Saumil S Shah; G Blair Martin Journal: Appl Environ Microbiol Date: 2010-03-19 Impact factor: 4.792
Authors: J P Wood; M W Calfee; M Clayton; N Griffin-Gatchalian; A Touati; S Ryan; L Mickelsen; L Smith; V Rastogi Journal: J Appl Microbiol Date: 2016-10-23 Impact factor: 3.772