Guoliang Xu1, Zengzhu Zhang, Zhuohui Zhang, Qiyun Zhang. 1. Jiangxi University of Traditional Chinese Medicine Modern Chinese Native Medicine Preparation Ministry of Education key laboratory, Nanchang 330004, China. xuguoliang6606@126.com
Abstract
OBJECTIVE: Characterizate the complete antigen of the chlorogenic acid. METHODS: The chlorogenic acid was coupled to BSA as an immunogen and to OVA as a coating antigen using the coupling agent EDC. HCL The immunogen and coating antigen were characterizated by the ultraviolet scanner. The complete antigen was used to immnune the cony pig, detect the antiserum by ELISA. RESULTS: The coupling ratio of the complete antigens was 20. The quinza pig antiserums in 1:128 in OD values were more higher than 2 times of the negative comparison. CONCLUSION: The complete antigen was preparated successfully.
OBJECTIVE: Characterizate the complete antigen of the chlorogenic acid. METHODS: The chlorogenic acid was coupled to BSA as an immunogen and to OVA as a coating antigen using the coupling agent EDC. HCL The immunogen and coating antigen were characterizated by the ultraviolet scanner. The complete antigen was used to immnune the cony pig, detect the antiserum by ELISA. RESULTS: The coupling ratio of the complete antigens was 20. The quinza pig antiserums in 1:128 in OD values were more higher than 2 times of the negative comparison. CONCLUSION: The complete antigen was preparated successfully.