| Literature DB >> 19238311 |
Frederick Pfister1, Yumei Wang, Kay Schreiter, Franziska vom Hagen, Karin Altvater, Sigrid Hoffmann, Urban Deutsch, Hans-Peter Hammes, Yuxi Feng.
Abstract
Our previous data suggested that angiopoietin-2 (Ang-2) is linked to pericyte loss, thereby playing an important role in diabetic retinopathy. In this study, we investigated the effect of retinal overexpression of human Ang-2 (mOpsinhAng2 mouse) on vascular morphology in non-diabetic and streptozotozin-induced diabetic animals. Pericyte (PC) coverage and acellular capillary (AC) formation were quantitated in retinal digest preparations after 3 and 6 months of diabetes duration. The degree of retinopathy in non-diabetic mOpsinhAng2 mice at 3 months (-21% PC, +49% AC) was comparable to age-matched diabetic wild type mice. Diabetic mOpsinhAng2 mice exhibited significantly worse vascular pathology than wild type counterparts at 6 months. Quantitative PCR revealed that human Ang-2 mRNA was highly overexpressed in retinas of transgenic mice. Our data demonstrate that overexpression of Ang-2 in the retina enhances vascular pathology, indicating that Ang-2 plays an essential role in diabetic vasoregression via destabilization of pericytes.Entities:
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Year: 2009 PMID： 19238311 DOI： 10.1007/s00592-009-0099-2
Source DB: PubMed Journal: Acta Diabetol ISSN： 0940-5429 Impact factor: 4.280