Debra A Carter1, Andrew D Dick, Eric J Mayer. 1. Academic Unit of Ophthalmology, Department of Clinical Sciences South Bristol, University of Bristol, Bristol Eye Hospital, Lower Maudlin Street, Bristol BS12LX, UK. debbie.carter@bristol.ac.uk
Abstract
BACKGROUND: CD133 is a cell surface marker of haematopoietic stem and progenitor cells. Leukaemia inhibitory factor (LIF), sustains proliferation and not differentiation of embryonic stem cells. We used CD133 to purify adult human retinal cells and aimed to determine what effect LIF had on these cultures and whether they still had the ability to generate neurospheres. METHODS: Retinal cell suspensions were derived from adult human post-mortem tissue with ethical approval. With magnetic automated cell sorting (MACS) CD133+ retinal cells were enriched from post mortem adult human retina. CD133+ retinal cell phenotype was analysed by flow cytometry and cultured cells were observed for proliferative capacity, neuropshere generation and differentiation with or without LIF supplementation. RESULTS: We demonstrated purification (to 95%) of CD133+ cells from adult human postmortem retina. Proliferating cells were identified through BrdU incorporation and expression of the proliferation markers Ki67 and Cyclin D1. CD133+ retinal cells differentiated whilst forming neurospheres containing appropriate lineage markers including glia, neurons and photoreceptors. LIF maintained CD133+ retinal cells in a proliferative and relatively undifferentiated state (Ki67, Cyclin D1 expression) without significant neurosphere generation. Differentiation whilst forming neurospheres was re-established on LIF withdrawal. CONCLUSION: These data support the evidence that CD133 expression characterises a population of cells within the resident adult human retina which have progenitor cell properties and that their turnover and differentiation is influenced by LIF. This may explain differences in retinal responses observed following disease or injury.
BACKGROUND:CD133 is a cell surface marker of haematopoietic stem and progenitor cells. Leukaemia inhibitory factor (LIF), sustains proliferation and not differentiation of embryonic stem cells. We used CD133 to purify adult human retinal cells and aimed to determine what effect LIF had on these cultures and whether they still had the ability to generate neurospheres. METHODS: Retinal cell suspensions were derived from adult human post-mortem tissue with ethical approval. With magnetic automated cell sorting (MACS) CD133+ retinal cells were enriched from post mortem adult human retina. CD133+ retinal cell phenotype was analysed by flow cytometry and cultured cells were observed for proliferative capacity, neuropshere generation and differentiation with or without LIF supplementation. RESULTS: We demonstrated purification (to 95%) of CD133+ cells from adult human postmortem retina. Proliferating cells were identified through BrdU incorporation and expression of the proliferation markers Ki67 and Cyclin D1. CD133+ retinal cells differentiated whilst forming neurospheres containing appropriate lineage markers including glia, neurons and photoreceptors. LIF maintained CD133+ retinal cells in a proliferative and relatively undifferentiated state (Ki67, Cyclin D1 expression) without significant neurosphere generation. Differentiation whilst forming neurospheres was re-established on LIF withdrawal. CONCLUSION: These data support the evidence that CD133 expression characterises a population of cells within the resident adult human retina which have progenitor cell properties and that their turnover and differentiation is influenced by LIF. This may explain differences in retinal responses observed following disease or injury.
Authors: Volkan Coskun; Hao Wu; Bruno Blanchi; Sean Tsao; Kevin Kim; Jing Zhao; Juan Carlos Biancotti; Leah Hutnick; Richard C Krueger; Guoping Fan; Jean de Vellis; Yi E Sun Journal: Proc Natl Acad Sci U S A Date: 2008-01-14 Impact factor: 11.205
Authors: Yunhe Xu; Balini Balasubramaniam; David A Copland; Jian Liu; M John Armitage; Andrew D Dick Journal: Graefes Arch Clin Exp Ophthalmol Date: 2015-02-14 Impact factor: 3.117
Authors: R A De Melo Reis; C S Schitine; A Köfalvi; S Grade; L Cortes; P F Gardino; J O Malva; F G de Mello Journal: Cell Mol Neurobiol Date: 2011-03-17 Impact factor: 5.046
Authors: József Jászai; Christine A Fargeas; Sylvi Graupner; Elly M Tanaka; Michael Brand; Wieland B Huttner; Denis Corbeil Journal: PLoS One Date: 2011-03-02 Impact factor: 3.240
Authors: J Lakowski; Y-T Han; R A Pearson; A Gonzalez-Cordero; E L West; S Gualdoni; A C Barber; M Hubank; R R Ali; J C Sowden Journal: Stem Cells Date: 2011-09 Impact factor: 6.277