Feeding high proportions of barley grain in a total mixed ration perturbs diurnal patterns of plasma metabolites in lactating dairy cows.

The aim of this study was to evaluate effects of feeding increasing proportions of barley grain in a total mixed ration (TMR) on diurnal plasma metabolite fluctuations in high-producing dairy cows. Eight early- to mid-lactation (60 to 140 d in milk) primiparous Holstein cows were assigned to a double 4 x 4 Latin square experimental design. Each experimental period lasted 21 d with the first 11 d used for diet adaptation. Cows were fed a TMR once daily at 0800 h containing no barley grain (control diet), or 15, 30, and 45% (dry matter basis) barley grain as well as barley silage. Blood samples were collected from the tail vein on the last day of each period shortly before (i.e., 0 h) and at 2, 4, 6, 8, 10, and 12 h after the morning feeding. Concentrations of glucose, nonesterified fatty acids (NEFA), beta-hydroxybutyric acid, cholesterol, and lactate in plasma were measured. Results of this study showed that feeding increasing proportions of barley grain affected concentrations of glucose and lactate in plasma with greater plasma glucose and lactate in cows fed the highest amount of grain; however, the amount of grain in the diet did not have an effect on diurnal patterns of plasma glucose. Additionally, the concentration of NEFA in plasma was greater in cows fed the higher grain diets and was greater in the hours following the morning meal than later in the day. The amount of grain in the diet was associated with lower plasma beta-hydroxybutyric acid, which increased particularly after the morning meal. Interestingly, cows fed the most barley grain had the lowest plasma cholesterol and this decreased during the day. In conclusion, the concomitant increase of glucose, lactate, and NEFA as well as the decrease of plasma cholesterol in cows fed high proportions of barley grain suggest that high inclusion of barley grain in the diet played a role in the diurnal patterns of plasma metabolites in lactating dairy cows. However, further research is warranted to understand involvement of these metabolic changes on the long-term health and productivity of dairy cows.