OBJECT: Abdominal aortic aneurysm (AAA) pathogenesis remains poorly understood. This study investigated the gene expression profile of peripheral blood from patients with AAA using microarray technology. METHODS AND RESULTS: We determined gene expression profiles in pooled RNA from 10 AAA patients and 10 matched controls with arrays representing 14,000 transcripts. Microarray data for selected genes were confirmed by real-time PCR in two different AAA (n=36) and control (n=36) populations and integrated with biochemical data. We identified 91 genes which were differentially expressed in AAA patients. Gene Ontology analysis indicated a significant alteration of oxygen transport (increased hemoglobin gene expression) and lipid metabolism [including monoglyceride lipase and low density lipoprotein receptor-related protein 5 (LRP5) gene]. LRP5 expression was associated inversely with serum lipoprotein(a) [Lp(a)] concentration. CONCLUSIONS: Increased expression of hemoglobin chain genes as well as of genes involved in erythrocyte mechanical stability were observed in the AAA RNA pools. The association between low levels of LRP5 gene expression and increased levels of Lp(a) in AAA patients suggests a potential role of LRP5 in Lp(a) catabolism. Our data underline the power of microarrays in identifying further molecular perturbations associated with AAA.
OBJECT: Abdominal aortic aneurysm (AAA) pathogenesis remains poorly understood. This study investigated the gene expression profile of peripheral blood from patients with AAA using microarray technology. METHODS AND RESULTS: We determined gene expression profiles in pooled RNA from 10 AAA patients and 10 matched controls with arrays representing 14,000 transcripts. Microarray data for selected genes were confirmed by real-time PCR in two different AAA (n=36) and control (n=36) populations and integrated with biochemical data. We identified 91 genes which were differentially expressed in AAA patients. Gene Ontology analysis indicated a significant alteration of oxygen transport (increased hemoglobin gene expression) and lipid metabolism [including monoglyceride lipase and low density lipoprotein receptor-related protein 5 (LRP5) gene]. LRP5 expression was associated inversely with serum lipoprotein(a) [Lp(a)] concentration. CONCLUSIONS: Increased expression of hemoglobin chain genes as well as of genes involved in erythrocyte mechanical stability were observed in the AAA RNA pools. The association between low levels of LRP5 gene expression and increased levels of Lp(a) in AAA patients suggests a potential role of LRP5 in Lp(a) catabolism. Our data underline the power of microarrays in identifying further molecular perturbations associated with AAA.
Authors: Shantel M Weinsheimer; Huichun Xu; Achal S Achrol; Boryana Stamova; Charles E McCulloch; Ludmila Pawlikowska; Yingfang Tian; Nerissa U Ko; Michael T Lawton; Gary K Steinberg; Steven D Chang; Glen Jickling; Bradley P Ander; Helen Kim; Frank R Sharp; William L Young Journal: Transl Stroke Res Date: 2011-12-01 Impact factor: 6.829
Authors: D Foer; M Zhu; R L Cardone; C Simpson; R Sullivan; S Nemiroff; G Lee; R G Kibbey; K F Petersen; K L Insogna Journal: Osteoporos Int Date: 2017-03-10 Impact factor: 4.507
Authors: Joseph V Moxon; Adam Parr; Theophilus I Emeto; Philip Walker; Paul E Norman; Jonathan Golledge Journal: Curr Probl Cardiol Date: 2010-10 Impact factor: 5.200
Authors: Bjorn Kloosterman; Marian Oortwijn; Jan uitdeWilligen; Twan America; Ric de Vos; Richard G F Visser; Christian W B Bachem Journal: BMC Genomics Date: 2010-03-08 Impact factor: 3.969
Authors: Joanna Pera; Michal Korostynski; Slawomir Golda; Marcin Piechota; Jaroslaw Dzbek; Tadeusz Krzyszkowski; Tomasz Dziedzic; Marek Moskala; Ryszard Przewlocki; Andrzej Szczudlik; Agnieszka Slowik Journal: J Cereb Blood Flow Metab Date: 2013-03-20 Impact factor: 6.200