Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Separation of protein oligomers by blue native gel electrophoresis.

Literature DB >> 19233117

Separation of protein oligomers by blue native gel electrophoresis.

Abstract

Native gel electrophoresis is used as a tool to assess structural differences in proteins. This article presents an application to separate oligomeric forms of proteins such as human immunodeficiency virus type 1 (HIV-1) reverse transcriptase monomers and homodimers. Technical difficulties encountered with various native gel techniques and ways to circumvent them are described.

Entities: Chemical Gene Mutation Species

Mesh：

Substances：

Year: 2009 PMID： 19233117 PMCID： PMC2898288 DOI： 10.1016/j.ab.2009.02.019

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

9 in total

1. Role of residues in the tryptophan repeat motif for HIV-1 reverse transcriptase dimerization.

Authors: Gilda Tachedjian; Hans-Erik G Aronson; Martha de los Santos; Jas Seehra; John M McCoy; Stephen P Goff
Journal: J Mol Biol Date: 2003-02-14 Impact factor: 5.469

2. Electrophoresis of proteins and protein-protein complexes in a native agarose gel.

Authors: R Kim; H Yokota; S H Kim
Journal: Anal Biochem Date: 2000-06-15 Impact factor: 3.365

3. Effects of efavirenz binding on the subunit equilibria of HIV-1 reverse transcriptase.

Authors: Carl F Venezia; Kathryn J Howard; Michael E Ignatov; Leslie A Holladay; Mary D Barkley
Journal: Biochemistry Date: 2006-03-07 Impact factor: 3.162

4. Blue native polyacrylamide gel electrophoresis (BN-PAGE) for the identification and analysis of multiprotein complexes.

Authors: Mahima Swamy; Gabrielle M Siegers; Susana Minguet; Bernd Wollscheid; Wolfgang W A Schamel
Journal: Sci STKE Date: 2006-07-25

5. Nonnucleoside reverse transcriptase inhibitors are chemical enhancers of dimerization of the HIV type 1 reverse transcriptase.

Authors: G Tachedjian; M Orlova; S G Sarafianos; E Arnold; S P Goff
Journal: Proc Natl Acad Sci U S A Date: 2001-06-19 Impact factor: 11.205

6. Alanine-scanning mutations in the "primer grip" of p66 HIV-1 reverse transcriptase result in selective loss of RNA priming activity.

Authors: M D Powell; M Ghosh; P S Jacques; K J Howard; S F Le Grice; J G Levin
Journal: J Biol Chem Date: 1997-05-16 Impact factor: 5.157

7. Analysis of molecular masses and oligomeric states of protein complexes by blue native electrophoresis and isolation of membrane protein complexes by two-dimensional native electrophoresis.

Authors: H Schägger; W A Cramer; G von Jagow
Journal: Anal Biochem Date: 1994-03 Impact factor: 3.365

8. Discontinuous native protein gel electrophoresis.

Authors: Michael Niepmann; Junfeng Zheng
Journal: Electrophoresis Date: 2006-10 Impact factor: 3.535

9. Separation of intact pyruvate dehydrogenase complex using blue native agarose gel electrophoresis.

Authors: N S Henderson; L G Nijtmans; J G Lindsay; E Lamantea; M Zeviani; I J Holt
Journal: Electrophoresis Date: 2000-08 Impact factor: 3.535

9 in total

6 in total

1. Efavirenz binding site in HIV-1 reverse transcriptase monomers.

Authors: Valerie A Braz; Mary D Barkley; Rebecca A Jockusch; Patrick L Wintrode
Journal: Biochemistry Date: 2010-11-19 Impact factor: 3.162

2. A modified clear-native polyacrylamide gel electrophoresis technique to investigate the oligomeric state of MBP-5-HT_3A-intracellular domain chimeras.

Authors: Akash Pandhare; Antonia G Stuebler; Elham Pirayesh; Michaela Jansen
Journal: Protein Expr Purif Date: 2018-08-18 Impact factor: 1.650