BACKGROUND: Recent studies demonstrate that low-level laser therapy (LLLT) modulates many biochemical processes, especially the decrease of muscle injures, the increase in mitochondrial respiration and ATP synthesis for accelerating the healing process. OBJECTIVE: In this work, we evaluated mitochondrial respiratory chain complexes I, II, III and IV and succinate dehydrogenase activities after traumatic muscular injury. METHODS: Male Wistar rats were randomly divided into three groups (n=6): sham (uninjured muscle), muscle injury without treatment, muscle injury with LLLT (AsGa) 5J/cm(2). Gastrocnemius injury was induced by a single blunt-impact trauma. LLLT was used 2, 12, 24, 48, 72, 96, and 120 hours after muscle-trauma. RESULTS: Our results showed that the activities of complex II and succinate dehydrogenase after 5days of muscular lesion were significantly increased when compared to the control group. Moreover, our results showed that LLLT significantly increased the activities of complexes I, II, III, IV and succinate dehydrogenase, when compared to the group of injured muscle without treatment. CONCLUSION: These results suggest that the treatment with low-level laser may induce an increase in ATP synthesis, and that this may accelerate the muscle healing process.
BACKGROUND: Recent studies demonstrate that low-level laser therapy (LLLT) modulates many biochemical processes, especially the decrease of muscle injures, the increase in mitochondrial respiration and ATP synthesis for accelerating the healing process. OBJECTIVE: In this work, we evaluated mitochondrial respiratory chain complexes I, II, III and IV and succinate dehydrogenase activities after traumatic muscular injury. METHODS: Male Wistar rats were randomly divided into three groups (n=6): sham (uninjured muscle), muscle injury without treatment, muscle injury with LLLT (AsGa) 5J/cm(2). Gastrocnemius injury was induced by a single blunt-impact trauma. LLLT was used 2, 12, 24, 48, 72, 96, and 120 hours after muscle-trauma. RESULTS: Our results showed that the activities of complex II and succinate dehydrogenase after 5days of muscular lesion were significantly increased when compared to the control group. Moreover, our results showed that LLLT significantly increased the activities of complexes I, II, III, IV and succinate dehydrogenase, when compared to the group of injured muscle without treatment. CONCLUSION: These results suggest that the treatment with low-level laser may induce an increase in ATP synthesis, and that this may accelerate the muscle healing process.
Authors: Larissa Aline Santos; Rodrigo Labat Marcos; Shaiane Silva Tomazoni; Adriane Aver Vanin; Fernanda Colella Antonialli; Vanessa dos Santos Grandinetti; Gianna Móes Albuquerque-Pontes; Paulo Roberto Vicente de Paiva; Rodrigo Álvaro Brandão Lopes-Martins; Paulo de Tarso Camillo de Carvalho; Jan Magnus Bjordal; Ernesto Cesar Pinto Leal-Junior Journal: Lasers Med Sci Date: 2014-03-21 Impact factor: 3.161
Authors: A M Clemente; L Rizzetto; G Castronovo; E Perissi; M Tanturli; F Cozzolino; D Cavalieri; F Fusi; F Cialdai; L Vignali; M G Torcia; M Monici Journal: Eur J Clin Microbiol Infect Dis Date: 2015-07-15 Impact factor: 3.267
Authors: José Carlos Tatmatsu-Rocha; Cleber Ferraresi; Michael R Hamblin; Flávio Damasceno Maia; Nilberto Robson Falcão do Nascimento; Patricia Driusso; Nivaldo Antonio Parizotto Journal: J Photochem Photobiol B Date: 2016-09-12 Impact factor: 6.252
Authors: Paulo V Mezzaroba; Dalton M Pessôa Filho; Alessandro M Zagatto; Fabiana Andrade Machado Journal: Lasers Med Sci Date: 2018-03-15 Impact factor: 3.161