| Literature DB >> 19231868 |
Kristene L Henne1, Joshua E Turse, Carrie D Nicora, Mary S Lipton, Sandra L Tollaksen, Carl Lindberg, Gyorgy Babnigg, Carol S Giometti, Cindy H Nakatsu, Dorothea K Thompson, Allan E Konopka.
Abstract
A global proteomic evaluation of the response of Arthrobacter sp. strain FB24 to 5 and 20 mM Cr(VI) was conducted using both two-dimensional gel electrophoresis (2-DGE) and liquid chromatography coupled with tandem mass spectrometry (LC/LC-MS/MS). The changes in protein expression found with 2-DGE indicate alterations in central metabolism and amino acid synthesis. Proteome coverage increased from 22% with 2-DGE to 71% with LC/LC-MS/MS. The proteins exhibiting the highest levels of expression under Cr(VI) stress suggest intracellular sulfur limitation, which could be driven by competition for the sulfate (SO4(2-)) transporter by the chromate (CrO4(2-)) ion. These results are consistent with the growth defects seen with strain FB24 when Cr(VI) concentrations exceeded 5 mM.Entities:
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Year: 2009 PMID： 19231868 DOI： 10.1021/pr800705f
Source DB: PubMed Journal: J Proteome Res ISSN： 1535-3893 Impact factor: 4.466