| Literature DB >> 19229761 |
Junfeng Liu1, Wen Zhang, Quan Shen, Shixing Yang, Fen Huang, Peifeng Li, Xuefeng Guo, Zhibiao Yang, Li Cui, Jianguo Zhu, Xiuguo Hua.
Abstract
In order to investigate the prevalence of HEV infection and immunity in pet dogs in the Jiang-Zhe area of China, we examined 192 dog serum samples collected from 11 animal hospitals in this area for anti-HEV IgG and HEV RNA, using ELISA and RT-PCR assays, respectively. Two dogs were injected intravenously with swine HEV to obtain anti-HEV antibody positive serum for ELISA assay. After the ELISA assay, 26 samples showing OD values higher than 0.20 were selected and examined by Dot-blot assay, and 88.5% (23/26) were confirmed to be positive for anti-HEV IgG. Although our results indicated swine HEV could experimentally induce anti-HEV IgG in the serum, we failed to detect HEV RNA in the serum samples from either the 2 intravenously injected dogs or 192 serum samples collected from animal hospitals. This implied that dog may not be a reservoir of HEV in this area.Entities:
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Year: 2009 PMID： 19229761 DOI： 10.1080/00365540902767031
Source DB: PubMed Journal: Scand J Infect Dis ISSN： 0036-5548