AIMS: To investigate the differences in membrane permeability and the effect on endocytosis of the polyene antimycotics nystatin, filipin and natamycin on germinating fungal conidia. METHODS AND RESULTS: The model system was Penicillium discolor, a food spoilage fungus. Filipin resulted in permeabilization of germinating conidia for the fluorescent probes TOTO-1 and FM4-64, but not for ferricyanide ions. Nystatin caused influx of all these compounds while natamycin did not. Untreated germinating conidia internalize the endocytic marker FM4-64. Pretreatment of germinating conidia with natamycin showed a dose and time dependent inhibition of endocytosis as judged by the lack of formation of early endosomal compartments. CONCLUSIONS: The results obtained from this study indicated that, unlike nystatin and filipin, natamycin is unable to permeabilize germinating conidia, but interferes with endocytosis in a dose and time dependent manner. SIGNIFICANCE AND IMPACT OF THE STUDY: Natamycin acts via a different mode of action than other polyene antimycotics. These results offer useful information for new strategies to prevent fungal spoilage on food products and infection on agricultural crops. For laboratory use, natamycin can be used as a specific inhibitor of early endocytosis in fungal cells.
AIMS: To investigate the differences in membrane permeability and the effect on endocytosis of the polyene antimycotics nystatin, filipin and natamycin on germinating fungal conidia. METHODS AND RESULTS: The model system was Penicillium discolor, a food spoilage fungus. Filipin resulted in permeabilization of germinating conidia for the fluorescent probes TOTO-1 and FM4-64, but not for ferricyanide ions. Nystatin caused influx of all these compounds while natamycin did not. Untreated germinating conidia internalize the endocytic marker FM4-64. Pretreatment of germinating conidia with natamycin showed a dose and time dependent inhibition of endocytosis as judged by the lack of formation of early endosomal compartments. CONCLUSIONS: The results obtained from this study indicated that, unlike nystatin and filipin, natamycin is unable to permeabilize germinating conidia, but interferes with endocytosis in a dose and time dependent manner. SIGNIFICANCE AND IMPACT OF THE STUDY: Natamycin acts via a different mode of action than other polyene antimycotics. These results offer useful information for new strategies to prevent fungal spoilage on food products and infection on agricultural crops. For laboratory use, natamycin can be used as a specific inhibitor of early endocytosis in fungal cells.
Authors: Yvonne Maria te Welscher; Lynden Jones; Martin Richard van Leeuwen; Jan Dijksterhuis; Ben de Kruijff; Gary Eitzen; Eefjan Breukink Journal: Antimicrob Agents Chemother Date: 2010-04-12 Impact factor: 5.191
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Authors: Jesús F Aparicio; Eva G Barreales; Tamara D Payero; Cláudia M Vicente; Antonio de Pedro; Javier Santos-Aberturas Journal: Appl Microbiol Biotechnol Date: 2015-10-29 Impact factor: 4.813
Authors: M R van Leeuwen; P Krijgsheld; T T Wyatt; E A Golovina; H Menke; A Dekker; J Stark; H Stam; R Bleichrodt; H A B Wösten; J Dijksterhuis Journal: Stud Mycol Date: 2012-09-24 Impact factor: 16.097
Authors: Frank J J Segers; Karel A van Laarhoven; Hendrik P Huinink; Olaf C G Adan; Han A B Wösten; Jan Dijksterhuis Journal: Appl Environ Microbiol Date: 2016-08-15 Impact factor: 4.792