| Literature DB >> 19226166 |
Nicoletta Bivi1, Jessica Z Bereszczak, Milena Romanello, Leo A H Zeef, Daniela Delneri, Franco Quadrifoglio, Luigi Moro, Francesco L Brancia, Gianluca Tell.
Abstract
We combined high-throughput screening of differential mRNAs with mass spectrometric characterization of proteins obtained from osteocytes untreated and treated with Risedronate. Microarray analysis revealed, upon treatment, a marked upregulation of messengers encoding zinc-proteins. MS analysis identified 84 proteins in the osteocytes proteome map. Risedronate affected the expression of 10 proteins, associated with cytoskeleton, stress-response and metabolism. Data validated using gel imaging in combination with the GLaD post digestion isotopic labeling method provide the molecular basis for understanding the role of bisphosphonates as antiapoptotic drugs for osteocytes.Entities:
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Year: 2009 PMID： 19226166 DOI： 10.1021/pr8005606
Source DB: PubMed Journal: J Proteome Res ISSN： 1535-3893 Impact factor: 4.466