Literature DB >> 19223635

Functional characterization of the sigmaB-dependent yabJ-spoVG operon in Staphylococcus aureus: role in methicillin and glycopeptide resistance.

Bettina Schulthess1, Stefan Meier, Dagmar Homerova, Christiane Goerke, Christiane Wolz, Jan Kormanec, Brigitte Berger-Bächi, Markus Bischoff.   

Abstract

The alternative sigma factor sigma(B) of Staphylococcus aureus controls the expression of multiple genes, including virulence determinants and global regulators; promotes capsule production; and increases the resistance levels of methicillin-resistant S. aureus (MRSA) and glycopeptide-intermediate-resistant S. aureus (GISA) strains. We show here that deletion of the sigma(B)-controlled yabJ-spoVG operon, which codes for potential downstream regulators of sigma(B), abolished capsule synthesis and reduced resistance in MRSA and GISA to the same extent that sigma(B) inactivation did. Introduction of the yabJ-spoVG operon in trans restored the original phenotype. By genetic manipulations, we show that SpoVG but not YabJ is required for complementation. We therefore postulate that SpoVG is the major factor of the yabJ-spoVG operon required in S. aureus for capsule formation and antibiotic resistance.

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Year:  2009        PMID： 19223635      PMCID： PMC2681525          DOI： 10.1128/AAC.01255-08

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  37 in total

1.  The alternative sigma factor sigmaB in Staphylococcus aureus: regulation of the sigB operon in response to growth phase and heat shock

Authors: 
Journal:  Arch Microbiol       Date:  1997-03-07       Impact factor: 2.552

2.  Conversion of a homogeneously methicillin-resistant strain of Staphylococcus aureus to heterogeneous resistance by Tn551-mediated insertional inactivation.

Authors:  J Kornblum; B J Hartman; R P Novick; A Tomasz
Journal:  Eur J Clin Microbiol       Date:  1986-12       Impact factor: 3.267

3.  Staphylococcal skin colonization in children with atopic dermatitis: prevalence, persistence, and transmission of toxigenic and nontoxigenic strains.

Authors:  P H Hoeger; W Lenz; A Boutonnier; J M Fournier
Journal:  J Infect Dis       Date:  1992-06       Impact factor: 5.226

4.  The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage.

Authors:  B N Kreiswirth; S Löfdahl; M J Betley; M O'Reilly; P M Schlievert; M S Bergdoll; R P Novick
Journal:  Nature       Date:  1983 Oct 20-26       Impact factor: 49.962

5.  Sigma(B) activity depends on RsbU in Staphylococcus aureus.

Authors:  P Giachino; S Engelmann; M Bischoff
Journal:  J Bacteriol       Date:  2001-03       Impact factor: 3.490

6.  In vitro transcription of pathogenesis-related genes by purified RNA polymerase from Staphylococcus aureus.

Authors:  L Rao; R K Karls; M J Betley
Journal:  J Bacteriol       Date:  1995-05       Impact factor: 3.490

7.  The sigma B regulon influences internalization of Staphylococcus aureus by osteoblasts.

Authors:  Sean P Nair; Markus Bischoff; Maria M Senn; Brigitte Berger-Bächi
Journal:  Infect Immun       Date:  2003-07       Impact factor: 3.441

8.  Impact of sigB mutation on Staphylococcus aureus oxacillin and vancomycin resistance varies with parental background and method of assessment.

Authors:  Vineet K Singh; Jennifer L Schmidt; R K Jayaswal; Brian J Wilkinson
Journal:  Int J Antimicrob Agents       Date:  2003-03       Impact factor: 5.283

9.  sigmaB modulates virulence determinant expression and stress resistance: characterization of a functional rsbU strain derived from Staphylococcus aureus 8325-4.

Authors:  Malcolm J Horsburgh; Joanne L Aish; Ian J White; Les Shaw; James K Lithgow; Simon J Foster
Journal:  J Bacteriol       Date:  2002-10       Impact factor: 3.490

10.  Optimization of a two-plasmid system for the identification of promoters recognized by RNA polymerase containing Staphylococcus aureus alternative sigma factor sigmaB.

Authors:  Dagmar Homerova; Markus Bischoff; Alexis Dumolin; Jan Kormanec
Journal:  FEMS Microbiol Lett       Date:  2004-03-19       Impact factor: 2.742
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  34 in total

1.  Transcriptional profiling of XdrA, a new regulator of spa transcription in Staphylococcus aureus.

Authors:  N McCallum; J Hinds; M Ender; B Berger-Bächi; P Stutzmann Meier
Journal:  J Bacteriol       Date:  2010-07-30       Impact factor: 3.490

2.  The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.

Authors:  Bettina Schulthess; Dominik A Bloes; Patrice François; Myriam Girard; Jacques Schrenzel; Markus Bischoff; Brigitte Berger-Bächi
Journal:  J Bacteriol       Date:  2011-07-01       Impact factor: 3.490

Review 3.  Antimicrobial resistance and virulence: a successful or deleterious association in the bacterial world?

Authors:  Alejandro Beceiro; María Tomás; Germán Bou
Journal:  Clin Microbiol Rev       Date:  2013-04       Impact factor: 26.132

Review 4.  Resilience in the Face of Uncertainty: Sigma Factor B Fine-Tunes Gene Expression To Support Homeostasis in Gram-Positive Bacteria.

Authors:  Claudia Guldimann; Kathryn J Boor; Martin Wiedmann; Veronica Guariglia-Oropeza
Journal:  Appl Environ Microbiol       Date:  2016-07-15       Impact factor: 4.792

5.  Strain-Specific Metabolic Requirements Revealed by a Defined Minimal Medium for Systems Analyses of Staphylococcus aureus.

Authors:  Henrique Machado; Liam L Weng; Nicholas Dillon; Yara Seif; Michelle Holland; Jonathan E Pekar; Jonathan M Monk; Victor Nizet; Bernhard O Palsson; Adam M Feist
Journal:  Appl Environ Microbiol       Date:  2019-10-16       Impact factor: 4.792

6.  Responses in the expression of extracellular proteins in methicillin-resistant Staphylococcus aureus treated with rhodomyrtone.

Authors:  Monton Visutthi; Potjanee Srimanote; Supayang Piyawan Voravuthikunchai
Journal:  J Microbiol       Date:  2011-12-28       Impact factor: 3.422

7.  New shuttle vector-based expression system to generate polyhistidine-tagged fusion proteins in Staphylococcus aureus and Escherichia coli.

Authors:  Sybille Schwendener; Vincent Perreten
Journal:  Appl Environ Microbiol       Date:  2015-03-06       Impact factor: 4.792

8.  Mutation of RNA polymerase beta subunit (rpoB) promotes hVISA-to-VISA phenotypic conversion of strain Mu3.

Authors:  Miki Matsuo; Tomomi Hishinuma; Yuki Katayama; Longzhu Cui; Maria Kapi; Keiichi Hiramatsu
Journal:  Antimicrob Agents Chemother       Date:  2011-07-11       Impact factor: 5.191

9.  Site-specific mutation of Staphylococcus aureus VraS reveals a crucial role for the VraR-VraS sensor in the emergence of glycopeptide resistance.

Authors:  Elena Galbusera; Adriana Renzoni; Diego O Andrey; Antoinette Monod; Christine Barras; Paolo Tortora; Alessandra Polissi; William L Kelley
Journal:  Antimicrob Agents Chemother       Date:  2010-12-20       Impact factor: 5.191

10.  VraT/YvqF is required for methicillin resistance and activation of the VraSR regulon in Staphylococcus aureus.

Authors:  Susan Boyle-Vavra; Shouhui Yin; Dae Sun Jo; Christopher P Montgomery; Robert S Daum
Journal:  Antimicrob Agents Chemother       Date:  2012-10-15       Impact factor: 5.191
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