| Literature DB >> 19223002 |
Ling Xu1, Xiujuan Qu, Ye Zhang, Xuejun Hu, Xianghong Yang, Kezuo Hou, Yuee Teng, Jingdong Zhang, Kiyonao Sada, Yunpeng Liu.
Abstract
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a member of the tumor necrosis factor family that selectively induces apoptosis in cancer cells. However, gastric cancer cells are insensitive to TRAIL. In the present study, we show that oxaliplatin enhanced TRAIL-induced apoptosis of MGC803, BGC823, and SGC7901 cells. Oxaliplatin promoted death receptor 4 (DR4) and death receptor 5 (DR5) clustering into aggregated lipid rafts, while the cholesterol-sequestering agent nystatin partially prevented lipid raft aggregation, DR4 and DR5 clustering, and reduced apoptosis. Furthermore, the expression of the casitas B-lineage lymphoma (Cbl) family was downregulated by oxaliplatin. Transfection of c-Cbl or Cbl-b partially reversed oxaliplatin-induced lipid raft aggregation. These results indicated that oxaliplatin enhanced TRAIL-induced gastric cancer cell apoptosis at least partially through Cbl-regulated death receptor redistribution in lipid rafts.Entities:
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Year: 2009 PMID： 19223002 DOI： 10.1016/j.febslet.2009.02.014
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124