| Literature DB >> 19219883 |
Yongqiang Cheng1, Xian Zhang, Zhengping Li, Xiaoxia Jiao, Yucong Wang, Yali Zhang.
Abstract
One-nucleotide differences in microRNAs (miRNAs) can be discriminated in an assay based on a branched rolling-circle amplification (BRCA) reaction and fluorescence quantification. With the proposed method miRNA can be detected at concentrations as low as 10 fM, and the miRNA in a total RNA sample of a few nanograms can be determined.Mesh:
Substances:
Year: 2009 PMID: 19219883 DOI: 10.1002/anie.200805665
Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN: 1433-7851 Impact factor: 15.336