BACKGROUND: Diabetic nephropathy represents a heterogeneous group of renal pathologies that may be associated with genetic susceptibility. There have been clinical reports on the risk association of diabetic nephropathy with an apolipoprotein E (ApoE) exon 4 polymorphism although its correlations with renal histopathological changes have not been explored. METHODS: A total of 213 adult autopsies with type 2 diabetes and 111 non-diabetic control cases were analysed. Genomic DNA samples were obtained from spleen tissues. The ApoE genotype was determined by PCR-LDR analysis. Histopathological examination of kidney sections was performed in a subset of 51 diabetic and 111 control cases. ApoE protein expression in diabetic carriers with similar clinical status was examined by immunohistochemical staining. RESULTS: In type 2 diabetes, epsilon2 carriers (P = 0.04; odds ratio = 5.42; 95% CI: 1.10-26.8) and epsilon3/epsilon4 (P = 0.04; odds ratio = 22.5; 95% CI: 1.11-454.90) genotype carriers were more likely to have glomerular hypertrophy than were epsilon3/epsilon3 carriers. The epsilon2 carriers showed an increase in glomerular ApoE protein expression. A correlation between ApoE genotype and nodular glomerulosclerosis was not found. CONCLUSIONS: Our findings confirm the risk association of the ApoE polymorphism with diabetic nephropathy in clinical studies and is the first study demonstrating the correlations between ApoE genotypes, protein expression and structural changes in diabetic nephropathy.
BACKGROUND:Diabetic nephropathy represents a heterogeneous group of renal pathologies that may be associated with genetic susceptibility. There have been clinical reports on the risk association of diabetic nephropathy with an apolipoprotein E (ApoE) exon 4 polymorphism although its correlations with renal histopathological changes have not been explored. METHODS: A total of 213 adult autopsies with type 2 diabetes and 111 non-diabetic control cases were analysed. Genomic DNA samples were obtained from spleen tissues. The ApoE genotype was determined by PCR-LDR analysis. Histopathological examination of kidney sections was performed in a subset of 51 diabetic and 111 control cases. ApoE protein expression in diabetic carriers with similar clinical status was examined by immunohistochemical staining. RESULTS: In type 2 diabetes, epsilon2 carriers (P = 0.04; odds ratio = 5.42; 95% CI: 1.10-26.8) and epsilon3/epsilon4 (P = 0.04; odds ratio = 22.5; 95% CI: 1.11-454.90) genotype carriers were more likely to have glomerular hypertrophy than were epsilon3/epsilon3 carriers. The epsilon2 carriers showed an increase in glomerular ApoE protein expression. A correlation between ApoE genotype and nodular glomerulosclerosis was not found. CONCLUSIONS: Our findings confirm the risk association of the ApoE polymorphism with diabetic nephropathy in clinical studies and is the first study demonstrating the correlations between ApoE genotypes, protein expression and structural changes in diabetic nephropathy.
Authors: Eliecer Coto; Juan Gómez; Beatriz Tavira; Salvador Tranche; Francisco Ortega; María I Rodríguez; Emilio Sánchez; Rafael Marín; Ana I Corao; Jorge Arenas; Victoria Alvarez Journal: Cardiorenal Med Date: 2013-05-16 Impact factor: 2.041
Authors: Ronald Ching Wan Ma; Heung Man Lee; Vincent Kwok Lim Lam; Claudia Ha Ting Tam; Janice Siu Ka Ho; Hai-Lu Zhao; Jing Guan; Alice Pik Shan Kong; Eric Lau; Guozhi Zhang; Andrea Luk; Ying Wang; Stephen Kwok Wing Tsui; Ting Fung Chan; Cheng Hu; Wei Ping Jia; Kyong Soo Park; Hong Kyu Lee; Hiroto Furuta; Kishio Nanjo; E Shyong Tai; Daniel Peng-Keat Ng; Nelson Leung Sang Tang; Jean Woo; Ping Chung Leung; Hong Xue; Jeffrey Wong; Po Sing Leung; Terrence C K Lau; Peter Chun Yip Tong; Gang Xu; Maggie Chor Yin Ng; Wing Yee So; Juliana Chung Ngor Chan Journal: PLoS One Date: 2014-01-20 Impact factor: 3.240