OBJECTIVE: To test the null hypothesis that increased tooth displacement in ovariectomized rats is not related to differential expressions of OPG and RANKL in the periodontium. MATERIALS AND METHODS: Eighty-four 12-week female rats were used; half were ovariectomized and half were not. Three months later, the maxillary first molar was moved mesially. Groups of rats were sacrificed at days 0, 1, 3, 5, 7, 10, and 14 after activation. Tooth movement was measured at each time point. OPG and RANKL expressions were examined through immunohistochemistry. RESULTS: Ovariectomized and nonovariectomized rats showed three-phase tooth movement. In both groups, OPG expression increased at the tension area and RANKL increased at the pressure area. The OPG/RANKL ratio coincided with tooth movement, especially in the linear phase from 7 to 14 days. CONCLUSIONS: The null hypothesis is rejected. The increased rate of tooth movement in ovariectomized rats was related to differential expressions of OPG and RANKL.
OBJECTIVE: To test the null hypothesis that increased tooth displacement in ovariectomized rats is not related to differential expressions of OPG and RANKL in the periodontium. MATERIALS AND METHODS: Eighty-four 12-week female rats were used; half were ovariectomized and half were not. Three months later, the maxillary first molar was moved mesially. Groups of rats were sacrificed at days 0, 1, 3, 5, 7, 10, and 14 after activation. Tooth movement was measured at each time point. OPG and RANKL expressions were examined through immunohistochemistry. RESULTS: Ovariectomized and nonovariectomized rats showed three-phase tooth movement. In both groups, OPG expression increased at the tension area and RANKL increased at the pressure area. The OPG/RANKL ratio coincided with tooth movement, especially in the linear phase from 7 to 14 days. CONCLUSIONS: The null hypothesis is rejected. The increased rate of tooth movement in ovariectomized rats was related to differential expressions of OPG and RANKL.
Authors: Felipe José Fernández-González; Aránzazu Cañigral; Felipe Balbontín-Ayala; José Manuel Gonzalo-Orden; Felix de Carlos; Teresa Cobo; Jose Pedro Fernández-Vázquez; Fernando Sánchez-Lasheras; José Antonio Vega Journal: Dental Press J Orthod Date: 2015-10
Authors: Basmah El-Sayed; Robert Philip Wynn Davies; Rehab R El-Zehery; Fatma Mohamed Ibrahim; Mohammed E Grawish; Jennifer Kirkham; Reem El-Gendy Journal: Front Bioeng Biotechnol Date: 2020-09-23