| Literature DB >> 19215882 |
Eldamária de Vargas Wolfgramm1, Fernanda Magri de Carvalho, Vitor Rezende da Costa Aguiar, Mariana Penha De Nadai Sartori, Gabriela C R Hirschfeld-Campolongo, Weslley M Tsutsumida, Iúri Drumond Louro.
Abstract
DNA isolation is the initial step of most genetic studies, and ideally it should use a reliable and non-invasive method. Buccal samples are adequate for such purposes, being painless, easy to collect and a very reliable DNA source. FTA Elute Cards are relatively new on the market and are designed for rapid blood DNA extraction, in which DNA is solubilized in water, instead of attached to the paper matrix. In this study, we sought to test if FTA Elute Cards are suitable for buccal DNA extraction. Furthermore, several time and temperature conditions were analyzed in order to determine the best concentration/time ratio. Twenty-five different conditions were tested in quadruplicate and extracted DNA was quantified by real-time PCR.Entities:
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Year: 2009 PMID： 19215882 DOI： 10.1016/j.fsigen.2008.11.008
Source DB: PubMed Journal: Forensic Sci Int Genet ISSN： 1872-4973 Impact factor: 4.882