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Literature DB >> 19215764

Chapter 14. Analysis of RNA-protein interactions using a yeast three-hybrid system.

Craig R Stumpf¹, Laura Opperman, Marvin Wickens.

Abstract

RNA-protein interactions play an essential role in the maturation and regulation of RNAs within eukaryotic organisms. The three-hybrid system provides a simple, yet powerful means to study RNA-protein interactions within the eukaryote Saccharomyces cerevisiae. This chapter describes the basis of the system and applications in both examining specific RNA-protein interactions and screening libraries for novel interactions. We provide a detailed discussion on affinity versus reporter output, variations on library screening (e.g., randomization studies), some adaptations of the system, and updated reagents and protocols.

Entities: Species

Mesh：

Substances：
RNA-Binding Proteins
RNA

Year: 2008 PMID： 19215764 DOI： 10.1016/S0076-6879(08)02414-2

Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600

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Cited

15 in total

1. Patterns and plasticity in RNA-protein interactions enable recruitment of multiple proteins through a single site.

Authors: Cary T Valley; Douglas F Porter; Chen Qiu; Zachary T Campbell; Traci M Tanaka Hall; Marvin Wickens
Journal: Proc Natl Acad Sci U S A Date: 2012-03-30 Impact factor: 11.205

Review 2. Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system.

Authors: Bram Stynen; Hélène Tournu; Jan Tavernier; Patrick Van Dijck
Journal: Microbiol Mol Biol Rev Date: 2012-06 Impact factor: 11.056

Chapter 14. Analysis of RNA-protein interactions using a yeast three-hybrid system.

1. Patterns and plasticity in RNA-protein interactions enable recruitment of multiple proteins through a single site.

Review 2. Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system.

3. A single C. elegans PUF protein binds RNA in multiple modes.

4. Stacking interactions in PUF-RNA complexes.

5. Specific and modular binding code for cytosine recognition in Pumilio/FBF (PUF) RNA-binding domains.

6. Ancestral Interactions of Ribosomal RNA and Ribosomal Proteins.

7. Alternative splicing regulation of doublesex gene by RNA-binding proteins in the silkworm Bombyx mori.

8. Context-dependent function of a conserved translational regulatory module.

9. Divergent RNA binding specificity of yeast Puf2p.

10. Treatment of type 1 myotonic dystrophy by engineering site-specific RNA endonucleases that target (CUG)(n) repeats.