Expression of Histamine HReceptors in Xenopus Oocytes Injected with Messenger Ribonucleic Acid from Bovine Adrenal Medulla: Pertussis Toxin Insensitive Activation of Membrane Chloride Currents.

Abstract Histamine H(2)-receptors have been identified in Xenopus oocytes previously microinjected with poly(A) + ribonucleic acid from bovine adrenal glands. Bath application of histamine to ribonucleic acid-primed oocytes evoked concentration-dependent, oscillating membrane currents under voltage-clamp conditions. H(1)-receptor specific antagonists clemastine, doxepin, pyrilamine, promethacine, diphenylhydramine, dephenylpyraline and chlorpheniramine, but not H(2)-receptor antagonists, cimetidine and ranitidine, inhibited histamine-induced responses. Membrane currents evoked by bath-applied histamine were insensitive to pertussis toxin, carried by chloride ions and dependent on intracellular but not extracellular calcium.