BACKGROUND: A recent genome-wide association study in adult patients with ulcerative colitis (UC) has implicated the interleukin 10 (IL-10) gene as an important candidate gene. Moreover, a UC-associated single nucleotide polymorphism (SNP) rs3024405 was also significantly associated with adult Crohn's disease (CD). AIMS: To examine whether IL-10-CD associations extended to paediatric-onset CD. METHODS: We implemented the case-control design at three paediatric gastroenterology clinics in Canada. CD patients (<or=20 years) were recruited along with healthy controls. DNA samples were genotyped for tag-single nucleotide polymorphisms (tag-SNPs) in the IL-10 gene. Allelic, genotype and haplotype associations with CD were studied. RESULTS: A total of 270 patients and 336 controls were studied. The mean age (+/-s.d.) at diagnosis was 12.1 (+/-3.5). There were a slightly higher proportion of male patients (56.3%). Of the five IL-10 tag-SNPs, rs2222202 (C/T) (P = 0.03) and rs1800871 (C/T) (P = 0.05) showed significant allelic associations with CD. Specific IL-10 SNPs were associated with CD disease location and/or disease behaviour. CONCLUSIONS: Our gene-wide analysis replicates recent findings of associations between IL-10 and adult CD, and suggests that these associations extend to paediatric-onset CD as well.
BACKGROUND: A recent genome-wide association study in adult patients with ulcerative colitis (UC) has implicated the interleukin 10 (IL-10) gene as an important candidate gene. Moreover, a UC-associated single nucleotide polymorphism (SNP) rs3024405 was also significantly associated with adult Crohn's disease (CD). AIMS: To examine whether IL-10-CD associations extended to paediatric-onset CD. METHODS: We implemented the case-control design at three paediatric gastroenterology clinics in Canada. CDpatients (<or=20 years) were recruited along with healthy controls. DNA samples were genotyped for tag-single nucleotide polymorphisms (tag-SNPs) in the IL-10 gene. Allelic, genotype and haplotype associations with CD were studied. RESULTS: A total of 270 patients and 336 controls were studied. The mean age (+/-s.d.) at diagnosis was 12.1 (+/-3.5). There were a slightly higher proportion of male patients (56.3%). Of the five IL-10 tag-SNPs, rs2222202 (C/T) (P = 0.03) and rs1800871 (C/T) (P = 0.05) showed significant allelic associations with CD. Specific IL-10 SNPs were associated with CD disease location and/or disease behaviour. CONCLUSIONS: Our gene-wide analysis replicates recent findings of associations between IL-10 and adult CD, and suggests that these associations extend to paediatric-onset CD as well.
Authors: Ryan M Huebinger; Fernando Rivera-Chavez; Ling-Yu Chang; Ming-Mei Liu; Joseph P Minei; Gary F Purdue; John L Hunt; Brett D Arnoldo; Robert C Barber Journal: J Surg Res Date: 2010-08-06 Impact factor: 2.192
Authors: Philip E Dubé; Fang Yan; Shivesh Punit; Nandini Girish; Steven J McElroy; M Kay Washington; D Brent Polk Journal: J Clin Invest Date: 2012-07-09 Impact factor: 14.808
Authors: Devendra K Amre; David R Mack; Kenneth Morgan; David Israel; Colette Deslandres; Ernest G Seidman; Phlippe Lambrette; Irina Costea; Alfreda Krupoves; Houda Fegury; Jinsong Dong; Zia Xhu; Guy Grimard; Emile Levy Journal: Hum Genet Date: 2010-05-16 Impact factor: 4.132
Authors: Yoshiyuki Mishima; Akihiko Oka; Bo Liu; Jeremy W Herzog; Chang Soo Eun; Ting-Jia Fan; Emily Bulik-Sullivan; Ian M Carroll; Jonathan J Hansen; Liang Chen; Justin E Wilson; Nancy C Fisher; Jenny Py Ting; Tomonori Nochi; Angela Wahl; J Victor Garcia; Christopher L Karp; R Balfour Sartor Journal: J Clin Invest Date: 2019-06-18 Impact factor: 14.808
Authors: Vibeke Andersen; Anja Ernst; Jane Christensen; Mette Østergaard; Bent A Jacobsen; Anne Tjønneland; Henrik B Krarup; Ulla Vogel Journal: BMC Med Genet Date: 2010-05-28 Impact factor: 2.103
Authors: Erik-Oliver Glocker; Daniel Kotlarz; Kaan Boztug; E Michael Gertz; Alejandro A Schäffer; Fatih Noyan; Mario Perro; Jana Diestelhorst; Anna Allroth; Dhaarini Murugan; Nadine Hätscher; Dietmar Pfeifer; Karl-Walter Sykora; Martin Sauer; Hans Kreipe; Martin Lacher; Rainer Nustede; Cristina Woellner; Ulrich Baumann; Ulrich Salzer; Sibylle Koletzko; Neil Shah; Anthony W Segal; Axel Sauerbrey; Stephan Buderus; Scott B Snapper; Bodo Grimbacher; Christoph Klein Journal: N Engl J Med Date: 2009-11-04 Impact factor: 91.245