Literature DB >> 19209350

Dynamic cell culture: a microfluidic function generator for live cell microscopy.

Philip J Lee1, Terry A Gaige, Paul J Hung.   

Abstract

We present a microfluidic system for time-lapsed, live cell microscopy with the ability to control solution exchange via a dynamic flow controller. The application specific microfluidic plates are designed to maintain adherent and non-adherent cell types for multiple days with continuous medium perfusion. Upstream channels with flow controlled via custom software allow the delivery of unique exposure profiles to the cultured cells, such as square waves, step functions, ramps, etc.

Entities:  

Mesh：

Year:  2008        PMID： 19209350     DOI： 10.1039/b807682k

Source DB:  PubMed          Journal:  Lab Chip        ISSN： 1473-0189            Impact factor:   6.799


  11 in total

1.  Linear conversion of pressure into concentration, rapid switching of concentration, and generation of linear ramps of concentration in a microfluidic device.

Authors:  Micha Adler; Alex Groisman
Journal:  Biomicrofluidics       Date:  2012-04-13       Impact factor: 2.800

Review 2.  Live-cell imaging of cyanobacteria.

Authors:  Rayka Yokoo; Rachel D Hood; David F Savage
Journal:  Photosynth Res       Date:  2014-11-04       Impact factor: 3.573

3.  Microfluidic system for generation of sinusoidal glucose waveforms for entrainment of islets of Langerhans.

Authors:  Xinyu Zhang; Alix Grimley; Richard Bertram; Michael G Roper
Journal:  Anal Chem       Date:  2010-08-01       Impact factor: 6.986

Review 4.  Microfluidic devices for measuring gene network dynamics in single cells.

Authors:  Matthew R Bennett; Jeff Hasty
Journal:  Nat Rev Genet       Date:  2009-08-11       Impact factor: 53.242

5.  Microfluidic multi-analyte gradient generator.

Authors:  Liaoran Cao; Xinyu Zhang; Alix Grimley; Anna R Lomasney; Michael G Roper
Journal:  Anal Bioanal Chem       Date:  2010-09-11       Impact factor: 4.142

6.  Magnetoactive sponges for dynamic control of microfluidic flow patterns in microphysiological systems.

Authors:  Sungmin Hong; Youngmee Jung; Ringo Yen; Hon Fai Chan; Kam W Leong; George A Truskey; Xuanhe Zhao
Journal:  Lab Chip       Date:  2013-12-06       Impact factor: 6.799

7.  Microfluidic single-cell array cytometry for the analysis of tumor apoptosis.

Authors:  Donald Wlodkowic; Shannon Faley; Michele Zagnoni; John P Wikswo; Jonathan M Cooper
Journal:  Anal Chem       Date:  2009-07-01       Impact factor: 6.986

8.  Vacuum-assisted cell loading enables shear-free mammalian microfluidic culture.

Authors:  Martin Kolnik; Lev S Tsimring; Jeff Hasty
Journal:  Lab Chip       Date:  2012-11-21       Impact factor: 6.799

Review 9.  Mycobacterial Growth.

Authors:  Iria Uhía; Kerstin J Williams; Vahid Shahrezaei; Brian D Robertson
Journal:  Cold Spring Harb Perspect Med       Date:  2015-05-08       Impact factor: 6.915

10.  A System for Analog Control of Cell Culture Dynamics to Reveal Capabilities of Signaling Networks.

Authors:  Chaitanya S Mokashi; David L Schipper; Mohammad A Qasaimeh; Robin E C Lee
Journal:  iScience       Date:  2019-08-08
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