| Literature DB >> 19205890 |
Longtao Zhang1, Wanmeng Mu, Bo Jiang, Tao Zhang.
Abstract
A non-characterized gene, previously proposed as the D-tagatose-3-epimerase gene from Rhodobacter sphaeroides, was cloned and expressed in Escherichia coli. Its molecular mass was estimated to be 64 kDa with two identical subunits. The enzyme specificity was highest with D-fructose and decreased for other substrates in the order: D-tagatose, D-psicose, D-ribulose, D-xylulose and D-sorbose. Its activity was maximal at pH 9 and 40 degrees C while being enhanced by Mn(2+). At pH 9 and 40 degrees C, 118 g D-psicose l(-1) was produced from 700 g D-fructose l(-1) after 3 h.Entities:
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Year: 2009 PMID： 19205890 DOI： 10.1007/s10529-009-9942-3
Source DB: PubMed Journal: Biotechnol Lett ISSN： 0141-5492 Impact factor: 2.461