| Literature DB >> 19205701 |
Egon Prochazka1, Tomas Soukup, Milos Hroch, Leos Fuksa, Eva Brcakova, Jolana Cermanova, Gabriela Kolouchova, Karel Urban, Jaroslav Mokry, Stanislav Micuda.
Abstract
Methotrexate (MTX) released from bone cement showed a useful local effect in animal models of bone tumours. However, local toxic reactions such as impaired wound healing were observed in areas surrounding the MTX-loaded implant. Therefore, we hypothesised that MTX released from bone cement would have harmful effects on human mesenchymal stem cells (MSC)-one of the basic components of bone marrow and tissue reparatory processes. Moreover, elution of MTX was calculated from implants prepared either with liquid or powdered MTX. During the 28-day incubation, the cement compounded with liquid MTX showed the highest elution rate of the drug. MTX released from pellets produced a significant decrease in proliferation of MSC as a consequence of a blockade of their cell cycle in the S/G2 phase. These findings indicate impairment of stem cell function in marginal areas surrounding the MTX-loaded cement and may help to explain problems with regeneration of tissues in these locations.Entities:
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Year: 2009 PMID： 19205701 PMCID： PMC2899262 DOI： 10.1007/s00264-008-0717-6
Source DB: PubMed Journal: Int Orthop ISSN： 0341-2695 Impact factor: 3.075