Using electrospray ionisation mass spectrometry to study non-covalent interactions.

The key strengths of electrospray over any other ionisation techniques are its soft nature and its ability to produce multiply charged ions. This combination is ideal for the study of non-covalent interactions. In this review article, we cover the basics of studying non-covalent interactions by mass spectrometry--illustrated with examples from our own and other labs--and discuss the current mass spectrometry based methods used for understanding and characterising non-covalent protein complexes.