Horseradish peroxidase immobilized on poly(thiophene-2-aminophenol-3-thiopheneacetic acid) film electrode with Au nanoparticle-fabrication and evaluation as hydrogen peroxide sensor.

Enzyme immobilized electrode was fabricated by two methods. In one of the methods, gold-nanoparticles (Au-NPs) prepared by gamma-irradiation were loaded into the copolymer film and horseradish peroxidase (HRP) was immobilized into the Au-NPs loaded copolymer film through physical entrapment. In the other method, the Au-NPs was prepared by electrochemical reduction of Au ions on the surface of poly(Th-AP-TAA) and HRP was immobilized into the Au-NPs. The enzyme immobilized electrodes were tested for electrocatalytic activities towards sensing of H2O2.