PURPOSE: Noninvasive in vivo cell-tracking techniques are necessary to advance the field of cellular-based therapeutics as well as to elucidate mechanisms governing in vivo cell biology. Fluorescence is commonly used for in vitro and postmortem biomedical studies but has been limited by autofluorescence at the whole-animal level. PROCEDURES: In this report, we demonstrate the ability of in vivo fluorescent lifetime imaging to remove autofluorescence and thereby enable in vivo dendritic cell tracking in naïve mice. Specifically, we track mature dendritic cells (DCs) labeled internally with near-infrared-emissive polymersomes (NIR-DCs). RESULTS: We establish the ability to detect labeled cells in vivo and image NIR-DC trafficking after both intravenous and subcutaneous delivery. In addition, we demonstrate the longitudinal capacity of this method by characterizing NIR-DC migration kinetics in the popliteal lymph node. CONCLUSIONS: This work provides a tool to evaluate dendritic-cell-based immunotherapy and generates novel opportunities for in vivo fluorescence imaging.
PURPOSE: Noninvasive in vivo cell-tracking techniques are necessary to advance the field of cellular-based therapeutics as well as to elucidate mechanisms governing in vivo cell biology. Fluorescence is commonly used for in vitro and postmortem biomedical studies but has been limited by autofluorescence at the whole-animal level. PROCEDURES: In this report, we demonstrate the ability of in vivo fluorescent lifetime imaging to remove autofluorescence and thereby enable in vivo dendritic cell tracking in naïve mice. Specifically, we track mature dendritic cells (DCs) labeled internally with near-infrared-emissive polymersomes (NIR-DCs). RESULTS: We establish the ability to detect labeled cells in vivo and image NIR-DC trafficking after both intravenous and subcutaneous delivery. In addition, we demonstrate the longitudinal capacity of this method by characterizing NIR-DC migration kinetics in the popliteal lymph node. CONCLUSIONS: This work provides a tool to evaluate dendritic-cell-based immunotherapy and generates novel opportunities for in vivo fluorescence imaging.
Authors: Lin Zhang; Nuo Yang; Jose-Ramon Conejo Garcia; Alisha Mohamed; Fabian Benencia; Stephen C Rubin; David Allman; George Coukos Journal: Am J Pathol Date: 2002-12 Impact factor: 4.307
Authors: M B Lappin; J M Weiss; V Delattre; B Mai; H Dittmar; C Maier; K Manke; S Grabbe; S Martin; J C Simon Journal: Immunology Date: 1999-10 Impact factor: 7.397
Authors: Xiuli Wang; Michael Rosol; Shundi Ge; Denise Peterson; George McNamara; Harvey Pollack; Donald B Kohn; Marvin D Nelson; Gay M Crooks Journal: Blood Date: 2003-08-28 Impact factor: 22.113
Authors: Shingo Baba; Steve Y Cho; Zhaohui Ye; Linzhao Cheng; James M Engles; Richard L Wahl Journal: Mol Imaging Date: 2007 Sep-Oct Impact factor: 3.250
Authors: Gregory P Robbins; Randi L Saunders; Jered B Haun; Jeff Rawson; Michael J Therien; Daniel A Hammer Journal: Langmuir Date: 2010-09-07 Impact factor: 3.882
Authors: Yasaman Ardeshirpour; Victor Chernomordik; Rafal Zielinski; Jacek Capala; Gary Griffiths; Olga Vasalatiy; Aleksandr V Smirnov; Jay R Knutson; Ilya Lyakhov; Samuel Achilefu; Amir Gandjbakhche; Moinuddin Hassan Journal: PLoS One Date: 2012-02-23 Impact factor: 3.240