AIM: We sought to determine the role of telomerase and its catalytic subunit hTERT in pancreatic cancer and evaluate the epigenetic regulation of hTERT by promoter methylation. METHODS: Thirty paired samples of pancreatic ductal adenocarcinomas and adjacent normal tissue and 12 chronic pancreatitis samples were studied. Reverse transcriptase polymerase chain reaction, telomeric repeat amplification protocol assay, and methylation-specific polymerase chain reaction were performed to analyze hTERT expression, telomerase activity, and methylation status of gene promoters, respectively. RESULT: hTERT and telomerase activity were upregulated in pancreatic cancer compared with paired normal tissues and samples of pancreatitis. hTERT expression correlated with telomerase activity (P \ .05) and in turn correlated positively with hTERT promoter methylation (P \ .001) and p16 promoter methylation. hTERT transcript expression and telomerase activity both conferred a worse outcome by univariate and multivariate analysis (P \ .05). CONCLUSION: hTERT expression and telomerase activity are predictors of poor outcome in pancreatic cancer. hTERT gene expression is positively regulated by promoter methylation.
AIM: We sought to determine the role of telomerase and its catalytic subunit hTERT in pancreatic cancer and evaluate the epigenetic regulation of hTERT by promoter methylation. METHODS: Thirty paired samples of pancreatic ductal adenocarcinomas and adjacent normal tissue and 12 chronic pancreatitis samples were studied. Reverse transcriptase polymerase chain reaction, telomeric repeat amplification protocol assay, and methylation-specific polymerase chain reaction were performed to analyze hTERT expression, telomerase activity, and methylation status of gene promoters, respectively. RESULT: hTERT and telomerase activity were upregulated in pancreatic cancer compared with paired normal tissues and samples of pancreatitis. hTERT expression correlated with telomerase activity (P \ .05) and in turn correlated positively with hTERT promoter methylation (P \ .001) and p16 promoter methylation. hTERT transcript expression and telomerase activity both conferred a worse outcome by univariate and multivariate analysis (P \ .05). CONCLUSION:hTERT expression and telomerase activity are predictors of poor outcome in pancreatic cancer. hTERT gene expression is positively regulated by promoter methylation.
Authors: Denise M Schütze; Jan M Kooter; Saskia M Wilting; Chris J L M Meijer; Wim Quint; Peter J F Snijders; Renske D M Steenbergen Journal: Epigenetics Date: 2015-01-23 Impact factor: 4.528
Authors: Alexandra S Shadrina; Uljana A Boyarskikh; Natalja A Oskina; Tatiana V Sinkina; Alexandr F Lazarev; Valentina D Petrova; Maxim L Filipenko Journal: Tumour Biol Date: 2014-10-10
Authors: Aaron S Mansfield; Liang Wang; Julie M Cunningham; Jin Jen; Christopher P Kolbert; Zhifu Sun; Ping Yang Journal: Cancer Genet Date: 2014-12-31
Authors: A G Schache; G Hall; J A Woolgar; G Nikolaidis; A Triantafyllou; D Lowe; J M Risk; R J Shaw; T Liloglou Journal: Br J Cancer Date: 2010-11-09 Impact factor: 7.640