| Literature DB >> 1919039 |
Y Fukuda1, J Hashino, M Haruyama, N Tsuruoka, H Nakazato, T Nakanishi.
Abstract
A sandwich enzyme-linked immunosorbent assay (ELISA) was developed for human interleukin-5 (hIL-5) using a combination of monoclonal anti-recombinant(r)-hIL-5 antibody and rabbit anti-r-hIL-5 IgG. Detection limit of this assay was estimated to be 7.8 pg/ml, which was about 10,000 times more sensitive than that of the bioassay using BCL1 cells of murine origin. This ELiSA was specific for hIL-5, showing no crossreactivity to recombinant human GM-CSF, IL-4, TNF-alpha, IFN-gamma and mouse IL-5 (mIL-5). The presence of 10% fetal calf serum did not interfere with the measurement of r-hIL-5. Coefficients of variation in intra-assay and interassay were 1.1-4.6% and 2.3-11.3%, respectively. These results indicate that this assay system can be quite useful in quantifying hIL-5 in various biological fluids.Entities:
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Year: 1991 PMID： 1919039 DOI： 10.1016/0022-1759(91)90276-l
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303