STUDY OBJECTIVES: To investigate whether noninvasive application of recurrent airway obstructions induces early release of mesenchymal stem cells into the circulating blood in a rat model of obstructive sleep apnea. DESIGN: Prospective controlled animal study. SETTING: University laboratory. PATIENTS OR PARTICIPANTS: Twenty male Sprague-Dawley rats (250-300 g). INTERVENTIONS: A specially designed nasal mask was applied to the anesthetized rats. Ten rats were subjected to a pattern of recurrent obstructive apneas (60 per hour, lasting 15 seconds each) for 5 hours. Ten anesthetized rats were used as controls. MEASUREMENTS AND RESULTS: Mesenchymal stem cells from the blood and bone marrow samples were isolated and cultured to count the total number of colony-forming unit fibroblasts (CFU-F) of adherent cells after 9 days in culture. The number of CFU-F from circulating blood was significantly (P = 0.02) higher in the rats subjected to recurrent obstructive apneas (5.00 +/- 1.16; mean +/- SEM) than in controls (1.70 +/- 0.72). No significant (P = 0.54) differences were observed in CFU-F from bone marrow. CONCLUSIONS: Application of a pattern of airway obstructions similar to those experienced by patients with sleep apnea induced an early mobilization of mesenchymal stem cells into circulating blood.
STUDY OBJECTIVES: To investigate whether noninvasive application of recurrent airway obstructions induces early release of mesenchymal stem cells into the circulating blood in a rat model of obstructive sleep apnea. DESIGN: Prospective controlled animal study. SETTING: University laboratory. PATIENTS OR PARTICIPANTS: Twenty male Sprague-Dawley rats (250-300 g). INTERVENTIONS: A specially designed nasal mask was applied to the anesthetized rats. Ten rats were subjected to a pattern of recurrent obstructive apneas (60 per hour, lasting 15 seconds each) for 5 hours. Ten anesthetized rats were used as controls. MEASUREMENTS AND RESULTS: Mesenchymal stem cells from the blood and bone marrow samples were isolated and cultured to count the total number of colony-forming unit fibroblasts (CFU-F) of adherent cells after 9 days in culture. The number of CFU-F from circulating blood was significantly (P = 0.02) higher in the rats subjected to recurrent obstructive apneas (5.00 +/- 1.16; mean +/- SEM) than in controls (1.70 +/- 0.72). No significant (P = 0.54) differences were observed in CFU-F from bone marrow. CONCLUSIONS: Application of a pattern of airway obstructions similar to those experienced by patients with sleep apnea induced an early mobilization of mesenchymal stem cells into circulating blood.
Authors: Jianguo Xu; Charles R Woods; Ana L Mora; Robert Joodi; Kenneth L Brigham; Smita Iyer; Mauricio Rojas Journal: Am J Physiol Lung Cell Mol Physiol Date: 2007-04-06 Impact factor: 5.464
Authors: Ramon Farré; Maria Nácher; Anna Serrano-Mollar; Juan B Gáldiz; Francisco J Alvarez; Daniel Navajas; Josep M Montserrat Journal: Sleep Date: 2007-07 Impact factor: 5.849
Authors: Mireia Dalmases; Marta Torres; Leonardo Márquez-Kisinousky; Isaac Almendros; Anna M Planas; Cristina Embid; Miguel Ángel Martínez-Garcia; Daniel Navajas; Ramon Farré; Josep Maria Montserrat Journal: Sleep Date: 2014-07-01 Impact factor: 5.849
Authors: Shaowei Li; Ke-Jung Huang; Jen-Chieh Wu; Michael S Hu; Mrinmoy Sanyal; Min Hu; Michael T Longaker; H Peter Lorenz Journal: Stem Cells Transl Med Date: 2015-03-05 Impact factor: 6.940
Authors: Isaac Almendros; Ramon Farré; Anna M Planas; Marta Torres; Maria R Bonsignore; Daniel Navajas; Josep M Montserrat Journal: Sleep Date: 2011-08-01 Impact factor: 5.849
Authors: Leila Kheirandish-Gozal; Rakesh Bhattacharjee; Jinkwan Kim; Heather B Clair; David Gozal Journal: Am J Respir Crit Care Med Date: 2010-03-04 Impact factor: 21.405
Authors: H Eimar; H Saltaji; S Ghorashi; D Isfeld; J E MacLean; D Gozal; D Graf; C Flores-Mir Journal: Osteoporos Int Date: 2017-01-18 Impact factor: 4.507