AIMS: To study the seasonal variation of Shiga toxin-encoding genes (stx) and to investigate the presence of Shiga toxin-producing Escherichia coli (STEC) O157 in cattle belonging to five dairy farms from Argentina. METHODS AND RESULTS: Rectal swab samples were collected from 360 dairy cows in each season and 115 and 137 calves in autumn and in spring, respectively. The stx were investigated by multiplex PCR and it was used as the indicator for STEC. Samples positives for stx were tested by PCR for eae-gamma1 of E. coli O157 and then subjected to IMS (immunomagnetic separation). In positive animals significant differences in the prevalence of stx between warm and cold seasons were detected. In warm seasons, stx1 + stx2 increased and stx1 decreased, independently of the animal category. The prevalence of STEC O157 in cows and calves were 0.2% and 0.8%, respectively. CONCLUSIONS: This work provides new data about the occurrence of stx and STEC O157 in dairy herds from Argentina and suggests a relationship between the type of stx and season of year. SIGNIFICANCE AND IMPACT OF STUDY: The detection of STEC O157 and the seasonality of stx and its types provide an opportunity to improve control strategies designed to prevent contamination of food products and transmission animal-person.
AIMS: To study the seasonal variation of Shiga toxin-encoding genes (stx) and to investigate the presence of Shiga toxin-producing Escherichia coli (STEC) O157 in cattle belonging to five dairy farms from Argentina. METHODS AND RESULTS: Rectal swab samples were collected from 360 dairy cows in each season and 115 and 137 calves in autumn and in spring, respectively. The stx were investigated by multiplex PCR and it was used as the indicator for STEC. Samples positives for stx were tested by PCR for eae-gamma1 of E. coli O157 and then subjected to IMS (immunomagnetic separation). In positive animals significant differences in the prevalence of stx between warm and cold seasons were detected. In warm seasons, stx1 + stx2 increased and stx1 decreased, independently of the animal category. The prevalence of STEC O157 in cows and calves were 0.2% and 0.8%, respectively. CONCLUSIONS: This work provides new data about the occurrence of stx and STEC O157 in dairy herds from Argentina and suggests a relationship between the type of stx and season of year. SIGNIFICANCE AND IMPACT OF STUDY: The detection of STEC O157 and the seasonality of stx and its types provide an opportunity to improve control strategies designed to prevent contamination of food products and transmission animal-person.
Authors: Giovanna I Andrade; Fernanda M Coura; Ethiene L S Santos; Marina G Ferreira; Grazielle C F Galinari; Elias J Facury Filho; Antônio U de Carvalho; Andrey P Lage; Marcos B Heinemann Journal: Trop Anim Health Prod Date: 2012-04-04 Impact factor: 1.559
Authors: Rosana Polifroni; Analía I Etcheverría; Marcelo E Sanz; Rosana E Cepeda; Alejandra Krüger; Paula M A Lucchesi; Daniel Fernández; Alberto E Parma; Nora L Padola Journal: Curr Microbiol Date: 2012-06-16 Impact factor: 2.188
Authors: Elisabetta Lambertini; Jeffrey S Karns; Jo Ann S Van Kessel; Huilin Cao; Ynte H Schukken; David R Wolfgang; Julia M Smith; Abani K Pradhan Journal: Appl Environ Microbiol Date: 2015-04-24 Impact factor: 4.792
Authors: Glen E Mellor; Thomas E Besser; Margaret A Davis; Brittany Beavis; Wookyung Jung; Helen V Smith; Amy V Jennison; Christine J Doyle; P Scott Chandry; Kari S Gobius; Narelle Fegan Journal: Appl Environ Microbiol Date: 2013-06-14 Impact factor: 4.792