Literature DB >> 19185710

Viability assays to monitor yeast autophagy.

Takeshi Noda1.   

Abstract

In the yeast Saccharomyces cerevisiae, autophagy contributes to the sustaining of cell viability under starvation conditions, possibly through the supply of amino acids that is generated as a result of the degradation of cytosolic materials. Therefore, cellular viability is one of the best indexes for monitoring the completion of the entire autophagic process. In this chapter, several assays for monitoring yeast viability are presented. Along with the standard colony-formation assay, assays using the dye phloxine B are introduced.

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Year:  2008        PMID： 19185710     DOI： 10.1016/S0076-6879(08)03202-3

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  15 in total

1.  A defect of the vacuolar putative lipase Atg15 accelerates degradation of lipid droplets through lipolysis.

Authors:  Yuichiro Maeda; Masahide Oku; Yasuyoshi Sakai
Journal:  Autophagy       Date:  2015       Impact factor: 16.016

2.  Survival in Quiescence Requires the Euchromatic Deployment of Clr4/SUV39H by Argonaute-Associated Small RNAs.

Authors:  Richard I Joh; Jasbeer S Khanduja; Isabel A Calvo; Meeta Mistry; Christina M Palmieri; Andrej J Savol; Shannan J Ho Sui; Ruslan I Sadreyev; Martin J Aryee; Mo Motamedi
Journal:  Mol Cell       Date:  2016-12-15       Impact factor: 17.970

3.  BAX inhibitor-1 regulates autophagy by controlling the IRE1α branch of the unfolded protein response.

Authors:  Karen Castillo; Diego Rojas-Rivera; Fernanda Lisbona; Benjamín Caballero; Melissa Nassif; Felipe A Court; Sebastian Schuck; Consuelo Ibar; Peter Walter; Jimena Sierralta; Alvaro Glavic; Claudio Hetz
Journal:  EMBO J       Date:  2011-09-16       Impact factor: 11.598

4.  Mitophagy plays an essential role in reducing mitochondrial production of reactive oxygen species and mutation of mitochondrial DNA by maintaining mitochondrial quantity and quality in yeast.

Authors:  Yusuke Kurihara; Tomotake Kanki; Yoshimasa Aoki; Yuko Hirota; Tetsu Saigusa; Takeshi Uchiumi; Dongchon Kang
Journal:  J Biol Chem       Date:  2011-12-07       Impact factor: 5.157

5.  Analysis of autophagy activated during changes in carbon source availability in yeast cells.

Authors:  Ryo Iwama; Yoshinori Ohsumi
Journal:  J Biol Chem       Date:  2019-02-12       Impact factor: 5.157

6.  Lipid droplets and their component triglycerides and steryl esters regulate autophagosome biogenesis.

Authors:  Tomer Shpilka; Evelyn Welter; Noam Borovsky; Nira Amar; Muriel Mari; Fulvio Reggiori; Zvulun Elazar
Journal:  EMBO J       Date:  2015-07-10       Impact factor: 11.598

7.  Fatty acid synthase is preferentially degraded by autophagy upon nitrogen starvation in yeast.

Authors:  Tomer Shpilka; Evelyn Welter; Noam Borovsky; Nira Amar; Frida Shimron; Yoav Peleg; Zvulun Elazar
Journal:  Proc Natl Acad Sci U S A       Date:  2015-01-20       Impact factor: 11.205

8.  RpkA, a highly conserved GPCR with a lipid kinase domain, has a role in phagocytosis and anti-bacterial defense.

Authors:  Tanja Y Riyahi; Frederike Frese; Michael Steinert; Napoleon N Omosigho; Gernot Glöckner; Ludwig Eichinger; Benoit Orabi; Robin S B Williams; Angelika A Noegel
Journal:  PLoS One       Date:  2011-11-02       Impact factor: 3.240

Review 9.  Guidelines and recommendations on yeast cell death nomenclature.

Authors:  Didac Carmona-Gutierrez; Maria Anna Bauer; Andreas Zimmermann; Andrés Aguilera; Nicanor Austriaco; Kathryn Ayscough; Rena Balzan; Shoshana Bar-Nun; Antonio Barrientos; Peter Belenky; Marc Blondel; Ralf J Braun; Michael Breitenbach; William C Burhans; Sabrina Büttner; Duccio Cavalieri; Michael Chang; Katrina F Cooper; Manuela Côrte-Real; Vítor Costa; Christophe Cullin; Ian Dawes; Jörn Dengjel; Martin B Dickman; Tobias Eisenberg; Birthe Fahrenkrog; Nicolas Fasel; Kai-Uwe Fröhlich; Ali Gargouri; Sergio Giannattasio; Paola Goffrini; Campbell W Gourlay; Chris M Grant; Michael T Greenwood; Nicoletta Guaragnella; Thomas Heger; Jürgen Heinisch; Eva Herker; Johannes M Herrmann; Sebastian Hofer; Antonio Jiménez-Ruiz; Helmut Jungwirth; Katharina Kainz; Dimitrios P Kontoyiannis; Paula Ludovico; Stéphen Manon; Enzo Martegani; Cristina Mazzoni; Lynn A Megeney; Chris Meisinger; Jens Nielsen; Thomas Nyström; Heinz D Osiewacz; Tiago F Outeiro; Hay-Oak Park; Tobias Pendl; Dina Petranovic; Stephane Picot; Peter Polčic; Ted Powers; Mark Ramsdale; Mark Rinnerthaler; Patrick Rockenfeller; Christoph Ruckenstuhl; Raffael Schaffrath; Maria Segovia; Fedor F Severin; Amir Sharon; Stephan J Sigrist; Cornelia Sommer-Ruck; Maria João Sousa; Johan M Thevelein; Karin Thevissen; Vladimir Titorenko; Michel B Toledano; Mick Tuite; F-Nora Vögtle; Benedikt Westermann; Joris Winderickx; Silke Wissing; Stefan Wölfl; Zhaojie J Zhang; Richard Y Zhao; Bing Zhou; Lorenzo Galluzzi; Guido Kroemer; Frank Madeo
Journal:  Microb Cell       Date:  2018-01-01

10.  Accelerated invagination of vacuoles as a stress response in chronically heat-stressed yeasts.

Authors:  Ayane Ishii; Masahito Kawai; Haruka Noda; Hiroyuki Kato; Kohei Takeda; Kotomi Asakawa; Yoshinobu Ichikawa; Tomohiro Sasanami; Keiji Tanaka; Yoko Kimura
Journal:  Sci Rep       Date:  2018-02-08       Impact factor: 4.379
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