| Literature DB >> 19185600 |
Olivier Terrier1, Jean-Paul Rolland, Manuel Rosa-Calatrava, Bruno Lina, Daniel Thomas, Vincent Moules.
Abstract
The knowledge of parainfluenza type 5 (PIV-5) virion morphology is essentially based on the observation of negatively stained preparations in conventional transmission electron microscopy (CTEM). In this study, the ultrastructure of frozen-hydrated intact PIV-5 was examined by cryo-electron microscopy (cryo-EM). Cryo-EM revealed a majority of spherical virions (70%), with a lower pleiomorphy than originally observed in CTEM. Phospholipid bilayer thickness, spike length and glycoprotein spikes density were measured. About 2000 glycoprotein spikes were present in an average-sized spherical virion. Altogether, these data depict a more precise view of PIV-5 morphology.Entities:
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Year: 2009 PMID： 19185600 DOI： 10.1016/j.virusres.2008.12.017
Source DB: PubMed Journal: Virus Res ISSN： 0168-1702 Impact factor: 3.303