Literature DB >> 19184988

Fluorescence detection of a protein-bound 2Fe2S cluster.

Kevin G Hoff1, Rochelle Goodlitt, Rui Li, Christina D Smolke, Jonathan J Silberg.   

Abstract

A fluorescent biosensor is described for 2Fe2S clusters that is composed of green fluorescent protein (GFP) fused to glutaredoxin 2 (Grx2), as illustrated here. 2Fe2S detection is based on the reduction of GFP fluorescence upon the 2Fe2S-induced dimerization of GFP-Grx2. This assay is sufficiently sensitive to detect submicromolar changes in 2Fe2S levels, thus making it suitable for high-throughput measurements of metallocluster degradation and synthesis reactions.

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Year:  2009        PMID： 19184988      PMCID： PMC3897124          DOI： 10.1002/cbic.200800747

Source DB:  PubMed          Journal:  Chembiochem        ISSN： 1439-4227            Impact factor:   3.164


  15 in total

1.  Multiple turnover transfer of [2Fe2S] clusters by the iron-sulfur cluster assembly scaffold proteins IscU and IscA.

Authors:  Francesco Bonomi; Stefania Iametti; Dennis Ta; Larry E Vickery
Journal:  J Biol Chem       Date:  2005-06-17       Impact factor: 5.157

Review 2.  Evolving strategies for enzyme engineering.

Authors:  Jesse D Bloom; Michelle M Meyer; Peter Meinhold; Christopher R Otey; Derek MacMillan; Frances H Arnold
Journal:  Curr Opin Struct Biol       Date:  2005-08       Impact factor: 6.809

3.  PRIM: proximity imaging of green fluorescent protein-tagged polypeptides.

Authors:  D A De Angelis; G Miesenböck; B V Zemelman; J E Rothman
Journal:  Proc Natl Acad Sci U S A       Date:  1998-10-13       Impact factor: 11.205

Review 4.  Iron-sulfur clusters: nature's modular, multipurpose structures.

Authors:  H Beinert; R H Holm; E Münck
Journal:  Science       Date:  1997-08-01       Impact factor: 47.728

5.  HscA and HscB stimulate [2Fe-2S] cluster transfer from IscU to apoferredoxin in an ATP-dependent reaction.

Authors:  Kala Chandramouli; Michael K Johnson
Journal:  Biochemistry       Date:  2006-09-19       Impact factor: 3.162

6.  Resonance Raman and magnetic circular dichroism studies of reduced [2Fe-2S] proteins.

Authors:  W Fu; P M Drozdzewski; M D Davies; S G Sligar; M K Johnson
Journal:  J Biol Chem       Date:  1992-08-05       Impact factor: 5.157

7.  IscU as a scaffold for iron-sulfur cluster biosynthesis: sequential assembly of [2Fe-2S] and [4Fe-4S] clusters in IscU.

Authors:  J N Agar; C Krebs; J Frazzon; B H Huynh; D R Dean; M K Johnson
Journal:  Biochemistry       Date:  2000-07-11       Impact factor: 3.162

8.  Characterization of human glutaredoxin 2 as iron-sulfur protein: a possible role as redox sensor.

Authors:  Christopher Horst Lillig; Carsten Berndt; Olivia Vergnolle; Maria Elisabet Lönn; Christoph Hudemann; Eckhard Bill; Arne Holmgren
Journal:  Proc Natl Acad Sci U S A       Date:  2005-05-25       Impact factor: 11.205

9.  Reversible sequestration of active site cysteines in a 2Fe-2S-bridged dimer provides a mechanism for glutaredoxin 2 regulation in human mitochondria.

Authors:  Catrine Johansson; Kathryn L Kavanagh; Opher Gileadi; Udo Oppermann
Journal:  J Biol Chem       Date:  2006-11-22       Impact factor: 5.157

10.  Splice mutation in the iron-sulfur cluster scaffold protein ISCU causes myopathy with exercise intolerance.

Authors:  Fanny Mochel; Melanie A Knight; Wing-Hang Tong; Dena Hernandez; Karen Ayyad; Tanja Taivassalo; Peter M Andersen; Andrew Singleton; Tracey A Rouault; Kenneth H Fischbeck; Ronald G Haller
Journal:  Am J Hum Genet       Date:  2008-02-14       Impact factor: 11.025
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  2 in total

1.  Fluorescent probes for tracking the transfer of iron-sulfur cluster and other metal cofactors in biosynthetic reaction pathways.

Authors:  James N Vranish; William K Russell; Lusa E Yu; Rachael M Cox; David H Russell; David P Barondeau
Journal:  J Am Chem Soc       Date:  2014-12-24       Impact factor: 15.419

Review 2.  Mitochondrial iron-sulfur cluster dysfunction in neurodegenerative disease.

Authors:  Grazia Isaya
Journal:  Front Pharmacol       Date:  2014-03-03       Impact factor: 5.810
  2 in total

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