OBJECTIVE AND DESIGN: Several studies report immuno-modulatory effects of endogenous IL-10 after trauma. This study investigates the effect of IL-10 administration on systemic and pulmonary inflammation in hemorrhagic shock. MATERIAL AND METHODS: Male C57/BL6 mice (4-6 animals per group) were subjected to volume controlled hemorrhagic shock for 3 hrs followed by resuscitation. Animals were either subcutaneously injected with 0.9 % saline (Shock group) or with recombinant mouse IL-10 (Shock+IL-10 group) 1 h before and 1 h after the induction of hemorrhagic shock. Serum TNF-alpha, IL-6, and keratinocyte (KC) concentrations were measured with the Luminex multiplexing platform. Acute pulmonary inflammation was assessed by pulmonary myeloperoxidase (MPO) and inducible nitric oxide synthase (iNOS) activity. RESULTS: IL-10 administration significantly decreased serum TNF-alpha (10.30 +/- 1.68 vs 37.42 +/- 10.64; p < 0.05), IL-6 (44.22 +/- 6.65 vs 85.24 +/- 7.94; p < 0.05), and KC (276.74 +/- 52.67 vs 465.61 +/- 58.98; p < 0.05) levels following hemorrhagic shock. Further, pulmonary MPO activity was significantly lower (2698.85 +/- 431.10 vs 4580.67 +/- 294.38; p < 0.05) and pulmonary iNOS activity upregulated. CONCLUSION: These findings suggest that administration of IL-10 modulates the degree of hemorrhage-induced systemic and pulmonary inflammation and support the notion of a central role for iNOS in acute lung injury.
OBJECTIVE AND DESIGN: Several studies report immuno-modulatory effects of endogenous IL-10 after trauma. This study investigates the effect of IL-10 administration on systemic and pulmonary inflammation in hemorrhagic shock. MATERIAL AND METHODS: Male C57/BL6 mice (4-6 animals per group) were subjected to volume controlled hemorrhagic shock for 3 hrs followed by resuscitation. Animals were either subcutaneously injected with 0.9 % saline (Shock group) or with recombinant mouseIL-10 (Shock+IL-10 group) 1 h before and 1 h after the induction of hemorrhagic shock. Serum TNF-alpha, IL-6, and keratinocyte (KC) concentrations were measured with the Luminex multiplexing platform. Acute pulmonary inflammation was assessed by pulmonary myeloperoxidase (MPO) and inducible nitric oxide synthase (iNOS) activity. RESULTS:IL-10 administration significantly decreased serum TNF-alpha (10.30 +/- 1.68 vs 37.42 +/- 10.64; p < 0.05), IL-6 (44.22 +/- 6.65 vs 85.24 +/- 7.94; p < 0.05), and KC (276.74 +/- 52.67 vs 465.61 +/- 58.98; p < 0.05) levels following hemorrhagic shock. Further, pulmonary MPO activity was significantly lower (2698.85 +/- 431.10 vs 4580.67 +/- 294.38; p < 0.05) and pulmonary iNOS activity upregulated. CONCLUSION: These findings suggest that administration of IL-10 modulates the degree of hemorrhage-induced systemic and pulmonary inflammation and support the notion of a central role for iNOS in acute lung injury.
Authors: Sergey B Zaets; Da-Zhong Xu; Qi Lu; Eleonora Feketova; Tamara L Berezina; Inga V Malinina; Edwin A Deitch; Eva H Olsen Journal: J Surg Res Date: 2010-12-31 Impact factor: 2.192
Authors: Neil R Aggarwal; Kenji Tsushima; Yoshiki Eto; Ashutosh Tripathi; Pooja Mandke; Jason R Mock; Brian T Garibaldi; Benjamin D Singer; Venkataramana K Sidhaye; Maureen R Horton; Landon S King; Franco R D'Alessio Journal: J Immunol Date: 2014-03-31 Impact factor: 5.422
Authors: Glendie Marcelin; Jerry R Aldridge; Susu Duan; Hazem E Ghoneim; Jerold Rehg; Henju Marjuki; Adrianus C M Boon; Jonathan A McCullers; Richard J Webby Journal: J Virol Date: 2011-08-24 Impact factor: 5.103
Authors: Aya M Westbrook; Bo Wei; Katrin Hacke; Menghang Xia; Jonathan Braun; Robert H Schiestl Journal: Mutagenesis Date: 2011-10-06 Impact factor: 3.000
Authors: Lei Sun; Elissa M Hult; Timothy T Cornell; Kevin K Kim; Thomas P Shanley; Carol A Wilke; Manisha Agarwal; Stephen J Gurczynski; Bethany B Moore; Mary K Dahmer Journal: Am J Physiol Lung Cell Mol Physiol Date: 2019-03-06 Impact factor: 5.464
Authors: Amy T Makley; Michael D Goodman; Lou Ann W Friend; Joseph S Deters; Jay A Johannigman; Warren C Dorlac; Alex B Lentsch; Timothy A Pritts Journal: J Trauma Date: 2010-02
Authors: Balázs Koscsó; Alexey Trepakov; Balázs Csóka; Zoltán H Németh; Pál Pacher; Holger K Eltzschig; György Haskó Journal: Purinergic Signal Date: 2013-04-13 Impact factor: 3.765