| Literature DB >> 19183659 |
M Gersbach1, D L Boiko, C Niclass, C C H Petersen, E Charbon.
Abstract
The fluorescence decay of high-affinity nonratiometric Ca2+ indicator Oregon Green BAPTA-1 (OGB-1) is analyzed with unprecedented temporal resolution in the two-photon excitation regime. A triple exponential decay is shown to best fit the fluorescence dynamics of OGB-1. We provide a model for accurate measurements of the free Ca2+ concentration and dissociation constants of nonratiometric calcium indicators.Entities:
Year: 2009 PMID: 19183659 DOI: 10.1364/ol.34.000362
Source DB: PubMed Journal: Opt Lett ISSN: 0146-9592 Impact factor: 3.776