UNLABELLED: The aim of this work is to evaluate the alterations of the oral ecosystem in symptomatic children with celiac disease (CD), to establish a particular pattern of oral markers that can be used as presumptive diagnosis of CD. MATERIAL & METHODS: A sample of n=52 children with CD diagnosis according to the modified criteria of the European Society of Pediatric Gastroenterology and Nutrition (ESPGAN), 1990, was studied. A dental clinical evaluation of soft and hard tissues was performed. Saliva samples were obtained; in which buffer capacity, total proteins, calcium and phosphate were measured and SDS PAGE 12% electrophoretic profiles were performed. In addition, oral mucosa smears were collected by brushing. RESULTS: Low frequency of enamel structural alterations was found, particularly in the permanent teeth of children with CD. These alterations had characteristics of chronological coherence (31.7%), bilateralism (26.8%) and symmetry (29.23%). The celiac smears in the celiac group (20%) showed signifcant presence of polymorphic nuclei and free nuclei. The celiac group had significant differences in buffer capacity, IgA levels, minute volume, calcium and Ca/P ratio (p<0.05). The protein profiles of CD children showed the absence of bands of low, medium and high molecular weight. CONCLUSION: Our results enable us to develop an alteration pattern corresponding to the oral ecosystem of CD children. In the CD patients, the most relevant variables were tooth enamel alterations, oral mucosa morphology, and modifications of salivary parameters, which would enable the dentist to refer these patients to specialist physician.
UNLABELLED: The aim of this work is to evaluate the alterations of the oral ecosystem in symptomatic children with celiac disease (CD), to establish a particular pattern of oral markers that can be used as presumptive diagnosis of CD. MATERIAL & METHODS: A sample of n=52 children with CD diagnosis according to the modified criteria of the European Society of Pediatric Gastroenterology and Nutrition (ESPGAN), 1990, was studied. A dental clinical evaluation of soft and hard tissues was performed. Saliva samples were obtained; in which buffer capacity, total proteins, calcium and phosphate were measured and SDSPAGE 12% electrophoretic profiles were performed. In addition, oral mucosa smears were collected by brushing. RESULTS: Low frequency of enamel structural alterations was found, particularly in the permanent teeth of children with CD. These alterations had characteristics of chronological coherence (31.7%), bilateralism (26.8%) and symmetry (29.23%). The celiac smears in the celiac group (20%) showed signifcant presence of polymorphic nuclei and free nuclei. The celiac group had significant differences in buffer capacity, IgA levels, minute volume, calcium and Ca/P ratio (p<0.05). The protein profiles of CDchildren showed the absence of bands of low, medium and high molecular weight. CONCLUSION: Our results enable us to develop an alteration pattern corresponding to the oral ecosystem of CDchildren. In the CDpatients, the most relevant variables were tooth enamel alterations, oral mucosa morphology, and modifications of salivary parameters, which would enable the dentist to refer these patients to specialist physician.
Authors: Ruggiero Francavilla; Danilo Ercolini; Maria Piccolo; Lucia Vannini; Sonya Siragusa; Francesca De Filippis; Ilaria De Pasquale; Raffaella Di Cagno; Michele Di Toma; Giorgia Gozzi; Diana I Serrazanetti; Maria De Angelis; Marco Gobbetti Journal: Appl Environ Microbiol Date: 2014-03-21 Impact factor: 4.792
Authors: Viviana Marisa Pereira Macho; Ana Sofia Coelho; Diana Maria Veloso E Silva; David José Casimiro de Andrade Journal: Open Dent J Date: 2017-10-24
Authors: Najlaa M Alamoudi; Farah A Alsadat; Azza A El-Housseiny; Osama M Felemban; Amani A Al Tuwirqi; Rana H Mosli; Omar I Saadah Journal: BMC Oral Health Date: 2020-11-09 Impact factor: 2.757
Authors: Katarzyna Marcinek; Rafał Wojciech Wójciak; Zbigniew Krejpcio; Maia Stanisławska-Kubiak Journal: Biol Trace Elem Res Date: 2015-08-21 Impact factor: 3.738