PURPOSE: We examined the potential for the pro-inflammatory complement proteins C5a and C3a to increase VEGF expression in ARPE-19 cells. MATERIALS AND METHODS: Expression of complement receptors in ARPE-19 cells was evaluated by RT-PCR. VEGF secretion from ARPE-19 cells treated with C5a or C3a was determined by ELISA. RESULTS: C5a and C3a receptor, but not C5L2, were detected in human eye tissue and ARPE-19 cells. C5a, but not C3a, treatment increased VEGF secretion from ARPE-19 cells, an effect inhibited by the C5aR antagonist, NDT 9513727. CONCLUSIONS: C5a receptor mediates increased VEGF secretion from ARPE-19 cells, suggesting a role for the C5a receptor in the pathogenesis of macular degeneration.
PURPOSE: We examined the potential for the pro-inflammatory complement proteins C5a and C3a to increase VEGF expression in ARPE-19 cells. MATERIALS AND METHODS: Expression of complement receptors in ARPE-19 cells was evaluated by RT-PCR. VEGF secretion from ARPE-19 cells treated with C5a or C3a was determined by ELISA. RESULTS:C5a and C3a receptor, but not C5L2, were detected in human eye tissue and ARPE-19 cells. C5a, but not C3a, treatment increased VEGF secretion from ARPE-19 cells, an effect inhibited by the C5aR antagonist, NDT 9513727. CONCLUSIONS:C5a receptor mediates increased VEGF secretion from ARPE-19 cells, suggesting a role for the C5a receptor in the pathogenesis of macular degeneration.
Authors: Ping Yang; Peter Baciu; Brittany C Parker Kerrigan; Menna Etheridge; Eric Sung; Brett A Toimil; Jacob E Berchuck; Glenn J Jaffe Journal: Invest Ophthalmol Vis Sci Date: 2014-05-06 Impact factor: 4.799
Authors: K Lueck; S Wasmuth; J Williams; T R Hughes; B P Morgan; A Lommatzsch; J Greenwood; S E Moss; D Pauleikhoff Journal: Eye (Lond) Date: 2011-05-20 Impact factor: 3.775
Authors: Selene Nunez-Cruz; Phyllis A Gimotty; Matthew W Guerra; Denise C Connolly; You-Qiang Wu; Robert A DeAngelis; John D Lambris; George Coukos; Nathalie Scholler Journal: Neoplasia Date: 2012-11 Impact factor: 5.715