Literature DB >> 19172344

Effect of low-level laser irradiation and epidermal growth factor on adult human adipose-derived stem cells.

B Mvula1, T J Moore, H Abrahamse.   

Abstract

The study investigated the effects of low-level laser radiation and epidermal growth factor (EGF) on adult adipose-derived stem cells (ADSCs) isolated from human adipose tissue. Isolated cells were cultured to semi-confluence, and the monolayers of ADSCs were exposed to low-level laser at 5 J/cm(2) using 636 nm diode laser. Cell viability and proliferation were monitored using adenosine triphosphate (ATP) luminescence and optical density at 0 h, 24 h and 48 h after irradiation. Application of low-level laser irradiation at 5 J/cm(2) on human ADSCs cultured with EGF increased the viability and proliferation of these cells. The results indicate that low-level laser irradiation in combination with EGF enhances the proliferation and maintenance of ADSCs in vitro.

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Year:  2009        PMID: 19172344     DOI: 10.1007/s10103-008-0636-1

Source DB:  PubMed          Journal:  Lasers Med Sci        ISSN: 0268-8921            Impact factor:   3.161


  30 in total

Review 1.  Adipose tissue-derived therapeutics.

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Review 5.  Epidermal growth factor.

Authors:  G Carpenter; S Cohen
Journal:  J Biol Chem       Date:  1990-05-15       Impact factor: 5.157

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9.  Increased survival of rat EGF-generated CNS precursor cells using B27 supplemented medium.

Authors:  C N Svendsen; J W Fawcett; C Bentlage; S B Dunnett
Journal:  Exp Brain Res       Date:  1995       Impact factor: 1.972

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  35 in total

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Review 4.  Adipose derived stem cells and smooth muscle cells: implications for regenerative medicine.

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Review 8.  The Effect of Photobiomodulation on Human Mesenchymal Cells: A Literature Review.

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