| Literature DB >> 19170029 |
Jason Bouvier1, Jr-Gang Cheng.
Abstract
Gene targeting in the mouse is an essential tool for studying gene function and creating models of human disease. The method described in this unit takes advantage of bacterial artificial chromosomes, Cre/loxP and FLPe/FRT systems, and recently evolved recombineering approaches to simplify the preparation of targeting constructs for generation of conditional knockout (CKO) animals. This method has been used to generate >30 CKO constructs, most of them successfully used to target mouse ES cells and establish mutant mice. Design and preparation of the CKO construct, as well as step-wise troubleshooting guidelines, are described in detail.Entities:
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Year: 2009 PMID： 19170029 DOI： 10.1002/0471142727.mb2313s85
Source DB: PubMed Journal: Curr Protoc Mol Biol ISSN： 1934-3647