Literature DB >> 19170021

Human iPS cell derivation/reprogramming.

In-Hyun Park1, George Q Daley.   

Abstract

This unit describes a protocol for deriving induced pluripotent stem (iPS) cells from human fibroblast cells. Human fibroblast cells are infected with retroviral vectors expressing four transcription factors (Oct4, Sox2, Klf4, and Myc) and selected for 3 to 4 weeks under human embryonic stem (hES) cell culture conditions. iPS cell colonies are mechanically isolated using a dissection microscope and handled like hES cells thereafter. Human iPS cells share similarities with hES cells including the expression of pluripotency genes, and differentiation as embryoid bodies in vitro into three germ layers (EB) and in vivo as teratomas.

Entities:  

Mesh：

Year:  2009        PMID： 19170021     DOI： 10.1002/9780470151808.sc04a01s8

Source DB:  PubMed          Journal:  Curr Protoc Stem Cell Biol        ISSN： 1938-8969


  13 in total

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10.  Excision of a viral reprogramming cassette by delivery of synthetic Cre mRNA.

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Journal:  Curr Protoc Stem Cell Biol       Date:  2012
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