OBJECTIVE: To evaluate the in vivo antimicrobial activity of different forms of application of chlorhexidine digluconate (CHX). METHOD AND MATERIALS: A group of 10 volunteers performed mouth rinsings with a 0.2% and 0.12% solution of CHX and applied CHX in the form of a 0.2% gel, a 0.2% and a 0.12% spray and a swab impregnated with a 0.2% solution. Samples of saliva were taken at baseline; 30 seconds; and 1, 3, 5, and 7 hours after each application. An epifluorescence microscopy technique (BacLight Live/Dead stain) was used to evaluate bacterial vitality. RESULTS:Bacterial vitality under basal conditions was 92%. At 30 seconds after CHX application, the lowest levels of viable bacteria were detected with the 0.2% and 0.12% solutions (1% and 8%, respectively). After 3 hours, the percentage of live bacteria achieved with the gel and sprays was similar to the basal figures (80% to 91%). At 7 hours, bacterial vitality recovered basal percentages, except with the 0.2% solution (77%). CONCLUSION: The 0.2% solution of CHX had the greatest sustained antimicrobial effect on the salivary flora, confirming the influence of the concentration and form of application on CHX substantivity.
RCT Entities:
OBJECTIVE: To evaluate the in vivo antimicrobial activity of different forms of application of chlorhexidine digluconate (CHX). METHOD AND MATERIALS: A group of 10 volunteers performed mouth rinsings with a 0.2% and 0.12% solution of CHX and applied CHX in the form of a 0.2% gel, a 0.2% and a 0.12% spray and a swab impregnated with a 0.2% solution. Samples of saliva were taken at baseline; 30 seconds; and 1, 3, 5, and 7 hours after each application. An epifluorescence microscopy technique (BacLight Live/Dead stain) was used to evaluate bacterial vitality. RESULTS: Bacterial vitality under basal conditions was 92%. At 30 seconds after CHX application, the lowest levels of viable bacteria were detected with the 0.2% and 0.12% solutions (1% and 8%, respectively). After 3 hours, the percentage of live bacteria achieved with the gel and sprays was similar to the basal figures (80% to 91%). At 7 hours, bacterial vitality recovered basal percentages, except with the 0.2% solution (77%). CONCLUSION: The 0.2% solution of CHX had the greatest sustained antimicrobial effect on the salivary flora, confirming the influence of the concentration and form of application on CHX substantivity.
Authors: Marcela R Carrilho; Ricardo M Carvalho; Ethan N Sousa; José Nicolau; Lorenzo Breschi; Annalisa Mazzoni; Leo Tjäderhane; Franklin R Tay; Kelli Agee; David H Pashley Journal: Dent Mater Date: 2010-05-15 Impact factor: 5.304
Authors: Jongryul Kim; Ryan M Vaughn; Lisha Gu; Roy A Rockman; Dwayne D Arola; Tara E Schafer; Kyoung Kyu Choi; David H Pashley; Franklin R Tay Journal: J Biomed Mater Res A Date: 2010-06-15 Impact factor: 4.396
Authors: Thaer Abouassi; Christian Hannig; Katja Mahncke; Lamprini Karygianni; Martin Wolkewitz; Elmar Hellwig; Ali Al-Ahmad Journal: BMC Res Notes Date: 2014-10-10