| Literature DB >> 19166842 |
Ya-Li Li1, Guang-Zhi Wu, Li Zeng, Gavin S Dawe, Li Sun, Gabriele Loers, Thomas Tilling, Shu-Sen Cui, Melitta Schachner, Zhi-Cheng Xiao.
Abstract
Cell surface glycosylation patterns are markers of cell type and status. However, the mechanisms regulating surface glycosylation patterns remain unknown. Using a panel of carbohydrate markers, we have shown that cell surface sialylation and fucosylation are upregulated in L1-transfected embryonic stem cells (L1-ESCs). Consistently, the mRNA levels of sialyltransferase ST6Gal1 and ST3Gal4, and fucosyltransferase FUT9 were significantly increased in L1-transfected ESCs. Activation of L1 signaling promoted cell survival and inhibited cell proliferation. ShRNAs knocking down FUT9, ST6Gal1 and ST3Gal4 blocked these effects. A phospholipase Cgamma (PLCgamma) inhibitor and shRNA reduced ST6Gal1, ST3Gal4 and FUT9 mRNA levels in the L1-ESCs. Thus, embryonic stem cell surface sialylation and fucosylation are regulated via PLCgamma by L1, with which they cooperate to modulate cell survival and proliferation.Entities:
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Year: 2009 PMID： 19166842 DOI： 10.1016/j.febslet.2009.01.013
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124