OBJECTIVE: To study whether levels of transrenal DNA (Tr-DNA) are influenced by the presence of urinary tract infections (UTI). METHODS: Tr-DNA concentrations were measured in 124 donors and 55 patients with UTI. Two methods for DNA extraction were compared. RESULTS: UTI patients showed higher concentrations than the donors (normal range: 0-147 GE/mL). QIAamp MiniElute Virus Spin Kit was more efficient than QIAamp DNA Blood Mini Kit. CONCLUSION: Increased concentrations of Tr-DNA urine DNA are shown in presence of UTI.
OBJECTIVE: To study whether levels of transrenal DNA (Tr-DNA) are influenced by the presence of urinary tract infections (UTI). METHODS: Tr-DNA concentrations were measured in 124 donors and 55 patients with UTI. Two methods for DNA extraction were compared. RESULTS: UTI patients showed higher concentrations than the donors (normal range: 0-147 GE/mL). QIAamp MiniElute Virus Spin Kit was more efficient than QIAamp DNA Blood Mini Kit. CONCLUSION: Increased concentrations of Tr-DNA urine DNA are shown in presence of UTI.
Authors: Sarah M Dermody; Chandan Bhambhani; Paul L Swiecicki; J Chad Brenner; Muneesh Tewari Journal: Front Genet Date: 2022-04-27 Impact factor: 4.772
Authors: Tuula K Outinen; Taru Kuparinen; Juulia Jylhävä; Sonja Leppänen; Jukka Mustonen; Satu Mäkelä; Ilkka Pörsti; Jaana Syrjänen; Antti Vaheri; Mikko Hurme Journal: PLoS One Date: 2012-02-07 Impact factor: 3.240