Literature DB >> 19157873

A rapid method for generation of selective Sox-based chemosensors of Ser/Thr kinases using combinatorial peptide libraries.

Juan A González-Vera1, Elvedin Luković, Barbara Imperiali.   

Abstract

A novel screening method to identify selective Sox-based fluorescent probes for Ser/Thr kinases has been developed. Peptide libraries were exposed to a kinase of interest and the products of the timed reaction were analyzed by MALDI-TOF. To demonstrate the potential of this methodology, a selective substrate for Aurora A kinase was identified that showed a 7-fold improvement in catalytic efficiency over the best substrate described to date in the literature.

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Year:  2008        PMID： 19157873      PMCID： PMC2838374          DOI： 10.1016/j.bmcl.2008.12.090

Source DB:  PubMed          Journal:  Bioorg Med Chem Lett        ISSN： 0960-894X            Impact factor:   2.823


  34 in total

Review 1.  Signaling--2000 and beyond.

Authors:  T Hunter
Journal:  Cell       Date:  2000-01-07       Impact factor: 41.582

Review 2.  Mitotic kinases as regulators of cell division and its checkpoints.

Authors:  E A Nigg
Journal:  Nat Rev Mol Cell Biol       Date:  2001-01       Impact factor: 94.444

Review 3.  Kinetic and catalytic mechanisms of protein kinases.

Authors:  J A Adams
Journal:  Chem Rev       Date:  2001-08       Impact factor: 60.622

Review 4.  Structural basis for control by phosphorylation.

Authors:  L N Johnson; R J Lewis
Journal:  Chem Rev       Date:  2001-08       Impact factor: 60.622

5.  Printing proteins as microarrays for high-throughput function determination.

Authors:  G MacBeath; S L Schreiber
Journal:  Science       Date:  2000-09-08       Impact factor: 47.728

6.  Phosphorylation of CPE binding factor by Eg2 regulates translation of c-mos mRNA.

Authors:  R Mendez; L E Hake; T Andresson; L E Littlepage; J V Ruderman; J D Richter
Journal:  Nature       Date:  2000-03-16       Impact factor: 49.962

Review 7.  Untangling the ErbB signalling network.

Authors:  Y Yarden; M X Sliwkowski
Journal:  Nat Rev Mol Cell Biol       Date:  2001-02       Impact factor: 94.444

8.  Peptide and protein library screening defines optimal substrate motifs for AKT/PKB.

Authors:  T Obata; M B Yaffe; G G Leparc; E T Piro; H Maegawa; A Kashiwagi; R Kikkawa; L C Cantley
Journal:  J Biol Chem       Date:  2000-11-17       Impact factor: 5.157

9.  Recognition-domain focused chemosensors: versatile and efficient reporters of protein kinase activity.

Authors:  Elvedin Luković; Juan A González-Vera; Barbara Imperiali
Journal:  J Am Chem Soc       Date:  2008-08-29       Impact factor: 15.419

10.  Aurora-A kinase is required for centrosome maturation in Caenorhabditis elegans.

Authors:  E Hannak; M Kirkham; A A Hyman; K Oegema
Journal:  J Cell Biol       Date:  2001-12-17       Impact factor: 10.539
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  8 in total

1.  A p38α-selective chemosensor for use in unfractionated cell lysates.

Authors:  Cliff I Stains; Elvedin Luković; Barbara Imperiali
Journal:  ACS Chem Biol       Date:  2010-09-30       Impact factor: 5.100

2.  Quantification of protein kinase enzymatic activity in unfractionated cell lysates using CSox-based sensors.

Authors:  Jon R Beck; Laura B Peterson; Barbara Imperiali; Cliff I Stains
Journal:  Curr Protoc Chem Biol       Date:  2014-09-09

3.  Bistability of a coupled Aurora B kinase-phosphatase system in cell division.

Authors:  Anatoly V Zaytsev; Dario Segura-Peña; Maxim Godzi; Abram Calderon; Edward R Ballister; Rumen Stamatov; Alyssa M Mayo; Laura Peterson; Ben E Black; Fazly I Ataullakhanov; Michael A Lampson; Ekaterina L Grishchuk
Journal:  Elife       Date:  2016-01-14       Impact factor: 8.140

4.  Synthesis of red-shifted 8-hydroxyquinoline derivatives using click chemistry and their incorporation into phosphorylation chemosensors.

Authors:  Juan A González-Vera; Elvedin Luković; Barbara Imperiali
Journal:  J Org Chem       Date:  2009-10-02       Impact factor: 4.354

5.  Selective mitogen activated protein kinase activity sensors through the application of directionally programmable D domain motifs.

Authors:  Laura B Peterson; Michael B Yaffe; Barbara Imperiali
Journal:  Biochemistry       Date:  2014-09-03       Impact factor: 3.162

Review 6.  Fluorescent Reporters and Biosensors for Probing the Dynamic Behavior of Protein Kinases.

Authors:  Juan A González-Vera; May C Morris
Journal:  Proteomes       Date:  2015-11-04

7.  Peptide-Peptide Co-Assembly: A Design Strategy for Functional Detection of C-peptide, A Biomarker of Diabetic Neuropathy.

Authors:  Kiat Hwa Chan; Jaehong Lim; Joo Eun Jee; Jia Hui Aw; Su Seong Lee
Journal:  Int J Mol Sci       Date:  2020-12-18       Impact factor: 5.923

8.  Enrichment of Aurora B kinase at the inner kinetochore controls outer kinetochore assembly.

Authors:  Mary Kate Bonner; Julian Haase; Jason Swinderman; Hyunmi Halas; Lisa M Miller Jenkins; Alexander E Kelly
Journal:  J Cell Biol       Date:  2019-09-16       Impact factor: 10.539
  8 in total

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