Season of the year influences semen output and concentrations of testosterone in circulation of yaks (Poephagus grunniens L.).

Seasonal changes in plasma testosterone concentration and semen quality were evaluated in yak bulls throughout a 1-year period. Blood samples were collected every week from adult yak bulls (n=15). These blood samples were analyzed for testosterone using a highly sensitive enzyme-linked immunoassay. Ejaculates were collected from five representative bulls each week. Ejaculate volume, progressive motility, live sperm count and sperm concentrations were determined. Mean testosterone in plasma was 1.03+/-0.25 ng/ml. Concentrations of testosterone changed throughout the year (P<0.05) and were found to be highest during the winter. It was also higher during the autumn than in summer and spring (P<0.05). Mean ejaculate volume, progressive motility, live sperm count and spermatozoa concentration were 2.7+/-0.3 ml, 72.8+/-1.4%, 82.3+/-0.9% and 968+/-233 x 10(6)ml(-1), respectively. Ejaculate volume and sperm concentration were higher (P<0.05) in autumn than in other seasons. To conclude, a highly sensitive EIA for testosterone was developed and validated for yak plasma. Seasonal changes in semen quality were associated with changes in the concentration of testosterone in plasma from yak bulls.